Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae

• Published in: Plant physiology (Bethesda) Vol. 111; no. 1; pp. 223 - 226
• Main Authors: Covello, P.S. (National Research Council, Saskatoon, Saskatchewan, Canada.), Reed, D.W
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.05.1996
• Subjects: ACIDE GRAS; ACIDOS GRASOS; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Analytical, structural and metabolic biochemistry; Arabidopsis - enzymology; Arabidopsis - genetics; ARABIDOPSIS THALIANA; Base Sequence; Biochemistry; Biochemistry and Enzymology; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; Biosynthesis; Cloning, Molecular; DNA Primers; Enzymes; Enzymes and enzyme inhibitors; EXPRESION GENICA; EXPRESSION DES GENES; Fatty Acid Desaturases - genetics; Fatty Acid Desaturases - metabolism; Fatty acids; Fundamental and applied biological sciences. Psychology; GENE; Gene expression; GENES; Genetic vectors; LECITHINE; LECITINAS; Lipids; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; OXIDORREDUCTASAS; OXYDOREDUCTASE; Plant cells; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; TRANSFERENCIA DE GENES; TRANSFERT DE GENE; Transformation, Genetic; Unsaturated fatty acids; Yeasts; Heterologous system; Enzyme; Phosphatidylcholine desaturase; Gene expression; Fatty acids; Linoleic acid; Fungi; Arabidopsis thaliana; Enzymatic activity; Cruciferae; Dicotyledones; Substrate specificity; Angiospermae; Ascomycetes; Spermatophyta; Oxidoreductases; Experimental plant; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.111.1.223

The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-olecyl-sn-glycero 3-phosphocholine A1 2-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and delta 9,delta 12-hexadecadienoic acid by gas chromatography-mass spectrometry