Striatal development involves a switch in gene expression networks, followed by a myelination event: Implications for neuropsychiatric disease

• Published in: Synapse (New York, N.Y.) Vol. 67; no. 4; pp. 179 - 188
• Main Authors: Novak, Gabriela, Fan, Theresa, O'dowd, Brian F., George, Susan R.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.04.2013; Wiley Subscription Services, Inc
• Subjects: Animals; dopamine; GABAergic Neurons - metabolism; gene expression; Gene Expression Profiling; Gene Expression Regulation, Developmental - physiology; Gene Regulatory Networks; medium spiny neurons; Models, Animal; Myelin Sheath - metabolism; Neostriatum - embryology; Neostriatum - growth & development; Neostriatum - metabolism; neurodevelopment; Rats; Rats, Sprague-Dawley; Receptors, Dopamine D1 - genetics; Receptors, Dopamine D1 - metabolism; Receptors, Dopamine D2 - genetics; Receptors, Dopamine D2 - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; schizophrenia; Schizophrenia - genetics; striatum; Synaptic Transmission - genetics
• ISSN: 0887-4476; 1098-2396
• DOI: 10.1002/syn.21628

Because abnormal development of striatal neurons is thought to be the part of pathology underlying major psychiatric illnesses, we studied the expression pattern of genes involved in striatal development and of genes comprising key striatal‐specific pathways, during an active striatal maturation period, the first two postnatal weeks in rat. This period parallels human striatal development during the second trimester, when prenatal stress is though to lead to increased risk for neuropsychiatric disorders. To identify genes involved in this developmental process, we used subtractive hybridization, followed by quantitative real‐time PCR, which allowed us to characterize the developmental expression of over 60 genes, many not previously known to play a role in neuromaturation. Of these 12 were novel transcripts, which did not match known genes, but which showed strict developmental expression and may play a role in striatal neurodevelopment. An additional 89 genes were identified as strong candidates for involvement in this neurodevelopmetnal process. We show that during the first two postnatal weeks in rat, an early gene expression network, still lacking key striatal‐specific signaling pathways, is downregulated and replaced by a mature gene expression network, containing key striatal‐specific genes including the dopamine D1 and D2 receptors, conferring to these neurons their functional identity. Therefore, before this developmental switch, striatal neurons lack many of their key phenotypic characteristics. This maturation process is followed by a striking rise in expression of myelination genes, indicating a striatal‐specific myelination event. Such strictly controlled developmental program has the potential to be a point of susceptibility to disruption by external factors. Indeed, this period is known to be a susceptibility period in both humans and rats. Synapse, 2013. © 2012 Wiley Periodicals, Inc.