Photolytic Protein Aggregates: Versatile Materials for Controlled Release of Active Proteins

Photolytic protein aggregates are developed as a facile and versatile platform for light-induced release of active proteins. The proteins modified with biotin through a photo-cleavable linker rapidly form aggregates with streptavidin and biotinylated functional molecules simply by mixing. Light irra...

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Bibliographic Details
Published inAdvanced healthcare materials Vol. 5; no. 9; p. 1002
Main Authors Ishiwatari, Akira, Yamaguchi, Satoshi, Takamori, Satoshi, Yamahira, Shinya, Minamihata, Kosuke, Nagamune, Teruyuki
Format Journal Article
LanguageEnglish
Published Germany 01.05.2016
Subjects
Cell Line
Delayed-Action Preparations - pharmacokinetics
Delayed-Action Preparations - pharmacology
Humans
Photolysis
Protein Aggregation, Pathological - drug therapy
Protein Aggregation, Pathological - metabolism
Protein Aggregation, Pathological - pathology
Transferrin - pharmacokinetics
Transferrin - pharmacology
protein engineering
mammalian cells
biomaterials;·drug delivery
photolysis
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Summary:Photolytic protein aggregates are developed as a facile and versatile platform for light-induced release of active proteins. The proteins modified with biotin through a photo-cleavable linker rapidly form aggregates with streptavidin and biotinylated functional molecules simply by mixing. Light irradiation releases active proteins from the aggregates in high yields, and light-induced uptake of drug-modified transferrin into living cells is successfully demonstrated.
ISSN:2192-2659
DOI:10.1002/adhm.201500957