Identification of amplified restriction fragment polymorphism (AFLP) markers tightly linked to the tomato Cf-9 gene for resistance to Cladosporium fulvum

• Published in: The Plant journal : for cell and molecular biology Vol. 8; no. 5; pp. 785 - 794
• Main Authors: Thomas, C.M, Vos, P, Zabeau, M, Jones, D.A, Norcott, K.A, Chadwick, B.P, Jones, J.D.G
• Format: Journal Article
• Language: English
• Published: Osney Mead, Oxford OX2 0EL, UK Blackwell Science Ltd 01.11.1995; Blackwell Science
• Subjects: Agronomy. Soil science and plant productions; Base Sequence; Biological and medical sciences; Chromosome Mapping; Cladosporium - pathogenicity; Cladosporium fulvum; Crosses, Genetic; crossing; disease resistance; DNA fingerprinting; f2 progeny; Fulvia fulva; Fundamental and applied biological sciences. Psychology; Gene Amplification; gene location; Genes, Plant; Genetic Linkage; Genetic Markers; genetic polymorphism; genetic recombination; Genetics and breeding of economic plants; Genomic Library; Immunity, Innate - genetics; Lycopersicon esculentum; Lycopersicon esculentum - genetics; Lycopersicon esculentum - microbiology; Meiosis; Molecular Sequence Data; Pest resistance; Plant Diseases - genetics; Plant pathogens; Polymorphism, Restriction Fragment Length; Recombination, Genetic; Solanum lycopersicum var. lycopersicum; Solanum pennellii var. pennellii; Species Specificity; Varietal selection. Specialized plant breeding, plant breeding aims; Genetic mapping; Cladosporium fulvum; Linkage; Plant pathogen; Genetic marker; Chromosome; Fungi; Microorganism resistance; Vegetable crop; Gene; Dicotyledones; Angiospermae; Lycopersicon esculentum; Spermatophyta; Fungi Imperfecti; Solanaceae; Thallophyta; Polymorphism
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.1995.08050785.x

Using the technique of amplified restriction fragment polymorphism (AFLP) analysis, and bulked segregant pools from F2 progeny of the cross Lycopersicon esculentum (Cf9) X L. pennellii, approximately 42 000 AFLP loci for tight linkage to the tomato Cf-9 gene for resistance to Cladosporium fulvum have been screened. Analysis of F2 recombinants identified three markers which co-segregated with Cf-9. The Cf-9 gene has recently been isolated by transposon tagging using the maize transposon Dissociation (Ds). Analysis of plasmid clones containing Cf-9 shows that two of these markers are located on opposite sides of the gene separated by 15.5 kbp of intervening DNA. AFLP analysis provides a rapid and efficient technique for detecting large numbers of DNA markers and should expedite plant gene isolation by positional cloning and the construction of high-density molecular linkage maps of plant genomes.