Nanoelectrode for Amperometric Monitoring of Individual Vesicular Exocytosis Inside Single Synapses

• Published in: Angewandte Chemie International Edition Vol. 53; no. 46; pp. 12456 - 12460
• Main Authors: Li, Yu-Tao, Zhang, Shu-Hui, Wang, Li, Xiao, Rong-Rong, Liu, Wei, Zhang, Xin-Wei, Zhou, Zhuan, Amatore, Christian, Huang, Wei-Hua
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 10.11.2014; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: amperometry; Animals; Cells, Cultured; complex exocytosis; Electrical measurements; Electrochemical Techniques - instrumentation; Electrochemistry; Endocrine glands; Equipment Design; Exocytosis; Fabrication; Fluxes; Mathematical analysis; Microelectrodes; Monitoring; nanoelectrodes; Nanostructure; Nanotechnology; Neurons - cytology; Neurons - metabolism; Neurotransmitter Agents - metabolism; Neurotransmitters; Real time; Superior Cervical Ganglion - cytology; Synapses; Synapses - metabolism; amperometry; synapses; complex exocytosis; neurotransmitters; nanoelectrodes

Chemical neurotransmission occurs at chemical synapses and endocrine glands, but up to now there was no means for direct monitoring of neurotransmitter exocytosis fluxes and their precise kinetics from inside an individual synapse. The fabrication of a novel finite conical nanoelectrode is reported...