Nanoelectrode for Amperometric Monitoring of Individual Vesicular Exocytosis Inside Single Synapses

Chemical neurotransmission occurs at chemical synapses and endocrine glands, but up to now there was no means for direct monitoring of neurotransmitter exocytosis fluxes and their precise kinetics from inside an individual synapse. The fabrication of a novel finite conical nanoelectrode is reported...

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Published inAngewandte Chemie International Edition Vol. 53; no. 46; pp. 12456 - 12460
Main Authors Li, Yu-Tao, Zhang, Shu-Hui, Wang, Li, Xiao, Rong-Rong, Liu, Wei, Zhang, Xin-Wei, Zhou, Zhuan, Amatore, Christian, Huang, Wei-Hua
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 10.11.2014
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
amperometry
Animals
Cells, Cultured
complex exocytosis
Electrical measurements
Electrochemical Techniques - instrumentation
Electrochemistry
Endocrine glands
Equipment Design
Exocytosis
Fabrication
Fluxes
Mathematical analysis
Microelectrodes
Monitoring
nanoelectrodes
Nanostructure
Nanotechnology
Neurons - cytology
Neurons - metabolism
Neurotransmitter Agents - metabolism
Neurotransmitters
Real time
Superior Cervical Ganglion - cytology
Synapses
Synapses - metabolism
amperometry
synapses
complex exocytosis
neurotransmitters
nanoelectrodes
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Summary:Chemical neurotransmission occurs at chemical synapses and endocrine glands, but up to now there was no means for direct monitoring of neurotransmitter exocytosis fluxes and their precise kinetics from inside an individual synapse. The fabrication of a novel finite conical nanoelectrode is reported perfectly suited in size and electrochemical properties for probing amperometrically inside what appears to be single synapses and monitoring individual vesicular exocytotic events in real time. This allowed obtaining direct and important physiological evidences which may yield important and new insights into the nature of synaptic communications. Chemical neurotransmission occurs at chemical synapse, but up to now there was no means for direct monitoring of neurotransmitter exocytosis and its precise kinetics from inside individual infinitesimal synapse. A novel finite conical nanoelectrode is fabricated and used in a newly developed amperometric method (see picture) for probing inside what appears to be single synapses.
Bibliography:ArticleID:ANIE201404744
ark:/67375/WNG-G9H9M3J0-G
National Natural Science Foundation of China - No. 21375099; No. 91017013
LIA CNRS XiamENS
Supported by the National Natural Science Foundation of China (grant numbers 21375099, 91017013), Doctoral Fund of Ministry of Education of China (grant number 20120141110031), the Program for Changjiang Scholars and Innovative Research Team in University (IRT1030), the Fundamental Research Funds for the Central Universities (grant number 2042014kf0192), UMR 8640 (CNRS-ENS-UPMC) and LIA CNRS XiamENS.
the Fundamental Research Funds for the Central Universities - No. 2042014kf0192
istex:EC5E57BDC6DA8385794E48470B75177BA539F6CB
Ministry of Education of China - No. 20120141110031
Program for Changjiang Scholars and Innovative Research Team in University - No. IRT1030
UMR 8640
Supported by the National Natural Science Foundation of China (grant numbers 21375099, 91017013), Doctoral Fund of Ministry of Education of China (grant number 20120141110031), the Program for Changjiang Scholars and Innovative Research Team in University (IRT1030), the Fundamental Research Funds for the Central Universities (grant number 2042014kf0192), UMR 8640 (CNRS‐ENS‐UPMC) and LIA CNRS XiamENS.
These authors contributed equally to this work.
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ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201404744