DNA methylation profiles provide a viable index for porcine pluripotent stem cells

Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor‐intensive and costly...

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Published inGenesis (New York, N.Y. : 2000) Vol. 51; no. 11; pp. 763 - 776
Main Authors Arai, Yoshikazu, Ohgane, Jun, Fujishiro, Shuh-hei, Nakano, Kazuaki, Matsunari, Hitomi, Watanabe, Masahito, Umeyama, Kazuhiro, Azuma, Dai, Uchida, Naomi, Sakamoto, Nozomu, Makino, Tomohiro, Yagi, Shintaro, Shiota, Kunio, Hanazono, Yutaka, Nagashima, Hiroshi
Format Journal Article
LanguageEnglish
Published United States Blackwell Publishing Ltd 01.11.2013
Wiley Subscription Services, Inc
BlackWell Publishing Ltd
Subjects
Animals
Blastocyst Inner Cell Mass - metabolism
Cell Line
Chimera
DNA Methylation
Embryo, Mammalian
Embryonic Stem Cells - metabolism
epigenetics
Gene Expression
Genes
Genetic Loci
induced pluripotent stem cells
Induced Pluripotent Stem Cells - metabolism
Mice
Swine - embryology
Swine, Miniature - embryology
Translational Medical Research
translational research
induced pluripotent stem cells
translational research
epigenetics
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Summary:Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor‐intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)‐specific hypomethylated loci (EShypo‐T‐DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso‐4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso‐4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso‐622‐14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum‐free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs. genesis 51:763–776. © 2013 Wiley Periodicals, Inc.
Bibliography:Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Young Scientists (B) (to J.O.), Contract grant sponsor: Research Project Grant (B) by Institute of Science and Technology Meiji University (to J.O.)
Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Agency (to Y.H. and H.N.), Contract grant sponsor: Meiji University International Institute for Bio-Resource Research (MUIIBR) (to H.N.)
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ArticleID:DVG22423
Meiji University Grant for Research by Young Scientist (to Y.A.)
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content type line 23
Contract grant sponsor: Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Agency (to Y.H. and H.N.), Contract grant sponsor: Meiji University International Institute for Bio-Resource Research (MUIIBR) (to H.N.); Contract grant sponsor: Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Young Scientists (B) (to J.O.), Contract grant sponsor: Research Project Grant (B) by Institute of Science and Technology Meiji University (to J.O.); Contract grant sponsor: Meiji University Grant for Research by Young Scientist (to Y.A.)
ISSN:1526-954X
1526-968X
DOI:10.1002/dvg.22423