Cadmium effect on oxidative metabolism of pea (Pisum sativum L.) roots. Imaging of reactive oxygen species and nitric oxide accumulation in vivo
ABSTRACT Growth of pea (Pisum sativum L.) plants with 50 µm CdCl2 for 15 d produced a reduction in the number and length of lateral roots, and changes in structure of the principal roots affecting the xylem vessels. Cadmium induced a reduction in glutathione (GSH) and ascorbate (ASC) contents, and c...
Saved in:
Published in | Plant, cell and environment Vol. 29; no. 8; pp. 1532 - 1544 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.08.2006
Blackwell |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | ABSTRACT
Growth of pea (Pisum sativum L.) plants with 50 µm CdCl2 for 15 d produced a reduction in the number and length of lateral roots, and changes in structure of the principal roots affecting the xylem vessels. Cadmium induced a reduction in glutathione (GSH) and ascorbate (ASC) contents, and catalase (CAT), GSH reductase (GR) and guaiacol peroxidase (GPX) activities. CuZn‐superoxide dismutase (SOD) activity was also diminished by the Cd treatment, although Mn‐SOD was slightly increased. CAT and CuZn‐SOD were down‐regulated at transcriptional level, while Mn‐SOD, Fe‐SOD and GR were up‐regulated. Analysis of reactive oxygen species (ROS) and nitric oxide (NO) levels by fluorescence and confocal laser microscopy (CLM) showed an over‐accumulation of O2.− and H2O2, and a reduction in the NO content in lateral and principal roots. ROS overproduction was dependent on changes in intracellular Ca+2 content, and peroxidases and NADPH oxidases were involved. Cadmium also produced an increase in salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) contents. The rise of ET and ROS, and the NO decrease are in accordance with senescence processes induced by Cd, and the increase of JA and SA could regulate the cellular response to cope with damages imposed by cadmium. |
---|---|
Bibliography: | Sequence data from this article has been deposited with the EMBL/GenBank data libraries under accession number AY426764. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0140-7791 1365-3040 |
DOI: | 10.1111/j.1365-3040.2006.01531.x |