紫果西番莲种子萌发及根部诱导愈伤组织研究

以西番莲种子为试验材料,在不同的赤霉素浸种时间及不同种子处理方式条件下,进行西番莲种子萌发试验,并利用发芽种子的根进行愈伤组织诱导。结果表明,将西番莲种子剥壳后置于MS培养基中萌发,是西番莲种子萌发的最适方式。根部愈伤组织诱导的最适培养基为MS＋0.5 mg/L 2,4-D,诱导出的愈伤组织多为浅绿色,颗粒状,质地疏松。...

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Published in西南农业学报 Vol. 26; no. 3; pp. 1148 - 1151
Main Author 张婷婷 吴田 张瑞丽 蓝增全
Format Journal Article
LanguageChinese
Published 西南林业大学环境科学与工程学院,云南昆明,650224%西南林业大学园林学院,云南昆明,650224%西南林业大学林学院,云南昆明,650224 2013
Subjects
愈伤组织
种子萌发
西番莲
Callus
Passiflora edulis
愈伤组织
西番莲
种子萌发
Seed germination
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ISSN1001-4829

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Summary:以西番莲种子为试验材料,在不同的赤霉素浸种时间及不同种子处理方式条件下,进行西番莲种子萌发试验,并利用发芽种子的根进行愈伤组织诱导。结果表明,将西番莲种子剥壳后置于MS培养基中萌发,是西番莲种子萌发的最适方式。根部愈伤组织诱导的最适培养基为MS＋0.5 mg/L 2,4-D,诱导出的愈伤组织多为浅绿色,颗粒状,质地疏松。
Bibliography:Passiflora edulis; Seed germination; Callus
51-1213/S
The germination rate of Passiflora edulis seed and the callus induction of root were studied in different seed soaking time with gibberellin and different seed treatment conditions.The results showed that the shucked Passiflora edulis seed on MS medium was the optimal way for seed germination.The optimal medium of root callus induction was MS＋0.5 mg/L 2,4-D,and the induced callus were mostly light green,granular and loose texture.
ZHANG Ting-ting1,WU Tian2＊,ZHANG Rui-li3,LAN Zeng-quan1（1.College of Environment Science and Engineering,Southwest Forestry University,Yunnan Kunming 650224,China;2.College of Horticulture and Gardening,Southwest Forestry University,Yunnan Kunming 650224,China;3.College of Forest,Southwest Forestry University,Yunnan Kunming 650224,China）
ISSN:1001-4829