栗疫菌营养体亲和型多样性比较及野生栗栗疫菌交配型的分子检测

在北京周边县区、湖北大老岭山区以及陕西安康地区分别调查栗疫病发生情况,采集病原栗疫菌（Cryphonec-tria parasitica）菌株并进行营养体亲和性试验。结果表明：北京板栗栽培区和陕西安康地区野生栗栗疫病发病率较高,局部地区危害严重;湖北大老岭山区栗疫病发病率低,危害轻微。北京地区菌株群体营养体亲和型多样性指数（Shannon-Wiener＇s diversity index）极显著低于湖北菌株群体和陕西菌株群体,而陕西与湖北菌株群体营养体亲和型多样性指数差异不显著。随机选取湖北、陕西野生栗栗疫菌部分菌株,利用特异性引物,通过普通PCR和巢氏PCR,对其交配型进行测定,发现陕西与湖...

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Published in林业科学研究 Vol. 25; no. 5; pp. 604 - 611
Main Author 潘琪 张国珍 贺伟 费松林 陈余朝 张海旺
Format Journal Article
LanguageChinese
Published 北京林业大学林学院,北京林业大学森林培育与保护教育部重点实验室,北京 100083%中国农业大学植物病理学系,农业部植物病理学实验室,北京100094%普渡大学林业与自然资源系,美国47907%陕西省安康市林业技术推广中心,陕西安康,725000 2012
Subjects
交配型
栗疫菌
营养体亲和型
野生栗
香农-维那指数
交配型
栗疫菌
香农-维那指数
野生栗
营养体亲和型
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Summary:在北京周边县区、湖北大老岭山区以及陕西安康地区分别调查栗疫病发生情况,采集病原栗疫菌（Cryphonec-tria parasitica）菌株并进行营养体亲和性试验。结果表明：北京板栗栽培区和陕西安康地区野生栗栗疫病发病率较高,局部地区危害严重;湖北大老岭山区栗疫病发病率低,危害轻微。北京地区菌株群体营养体亲和型多样性指数（Shannon-Wiener＇s diversity index）极显著低于湖北菌株群体和陕西菌株群体,而陕西与湖北菌株群体营养体亲和型多样性指数差异不显著。随机选取湖北、陕西野生栗栗疫菌部分菌株,利用特异性引物,通过普通PCR和巢氏PCR,对其交配型进行测定,发现陕西与湖北野生栗菌株群体中均存在MAT-1与MAT-2两种交配型的菌株。通过PCR扩增,在两地的野生菌株群体里均发现同时具有两种交配型基因的菌株,其中,陕西群体此类菌株比例较大,湖北菌株比例较小
Bibliography:11-1221/S
PAN Qi,ZHANG Guo-zhen,HE Wei,FEI Song-lin,CHEN Yu-chao,ZHANG Hai-wang（1.The Academy of Forestry,Beijing Forestry University,Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing 100083,China;2.Department of Plant Pathology,College of Agriculture and Biotechnology,China Agricultural University, Key Laboratory of Plant Pathology,Ministry of Agriculture,Beijing 100094,China; 3.Department of Forestry and Natural Resource,Purdue University,West Lafayette 47907,USA; 4.Forestry Technology Promotion Center of Ankang City,Ankang 725000,Shaanxi,China）
The incidence of chestnut blight caused by Cryphonectria parasitica in the regions of Beijing,Hubei and Shaanxi was investigated and the pathogen strains collected from these regions were used to detect their vegetative compatibility groups（VCGs）.The results showed that the incidence of chestnut blight in Beijing and Shaanxi,varying according to different site conditions,was higher than that in Hubei.The Shannon-Wiener＇s diversity inde
ISSN:1001-1498