一株降解玉米秸秆菌株的分离、鉴定及其降解能力的研究

从玉米秸秆上分离筛选出的S-3菌株能够分泌纤维素酶,具有降解农作物秸秆的能力。该菌经鉴定为芽孢杆菌属,其产纤维素酶活为172.786 U/g,最佳降解条件为发酵时间11 d、发酵温度32℃、料水比1∶2.5、S-3菌株用量10 mL。在上述条件下,纤维素占发酵干基的比率从最初的35.48%降到23.26%;木质素从最初的15.37%降到14.51%。...

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Bibliographic Details
Published in广东农业科学 Vol. 39; no. 20; pp. 87 - 90
Main Author 周沅洁 马桂英 王修俊 艾静汶 朱隆绘
Format Journal Article
LanguageChinese
Published 贵州大学化学与化工学院,贵州贵阳550025 2012
贵州省发酵工程与生物制药重点实验室,贵州贵阳550025%贵州大学化学与化工学院,贵州贵阳,550025
Subjects
分离筛选
秸秆
鉴定
降解能力
秸秆
分离筛选
鉴定
降解能力
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ISSN1004-874X

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Summary:从玉米秸秆上分离筛选出的S-3菌株能够分泌纤维素酶,具有降解农作物秸秆的能力。该菌经鉴定为芽孢杆菌属,其产纤维素酶活为172.786 U/g,最佳降解条件为发酵时间11 d、发酵温度32℃、料水比1∶2.5、S-3菌株用量10 mL。在上述条件下,纤维素占发酵干基的比率从最初的35.48%降到23.26%;木质素从最初的15.37%降到14.51%。
Bibliography:44-1267/S
ZHOU Yuan-jie,MA Gui-ying,WANG Xiu-jun,AI Jing-wen,ZHU Long-hui（1.College of Chemistry and Chemical Engineering,Guizhou University,Guiyang 550025,China; 2.Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy,Guiyang 550025,China）
In this study,we isolated strains from the corn straw which could secrete cellulose and degraded straw.This bacteria was identified as Bacillus,and its cellulase activity was 172.786 U/g.The best degradation conditions were as follows： fermentation time of 11 days,culture temperature 32℃,culture medium material ratio 1∶2.5,inoculation of strains 10 mL.Under these conditions,the proportion of cellulose dropped from 35.48% to 23.26%,and the lignin dropped from 15.37% to 14.51%.
straw; degradation capability; isolation
ISSN:1004-874X