Porphyromonas gingivalis Produce Neutrophil Specific Chemoattractants Including Short Chain Fatty Acids

• Published in: Frontiers in cellular and infection microbiology Vol. 10; p. 620681
• Main Authors: Dahlstrand Rudin, Agnes, Khamzeh, Arsham, Venkatakrishnan, Vignesh, Persson, Tishana, Gabl, Michael, Savolainen, Otto, Forsman, Huamei, Dahlgren, Claes, Christenson, Karin, Bylund, Johan
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 19.01.2021
• Subjects: acetate; Cellular and Infection Microbiology; Chemotactic Factors; Fatty Acids, Volatile; FFAR2; GPR43; granulocyte; Immunologi inom det medicinska området; Immunology in the medical area; Interleukin-8; Microbiology in the medical area; Mikrobiologi inom det medicinska området; Neutrophils; periodontitis; polymorphonuclear leucocyte; Porphyromonas gingivalis; propionate; short chain fatty acid; acetate; granulocyte; polymorphonuclear leucocyte; GPR43; periodontitis; FFAR2; propionate; short chain fatty acid
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2020.620681

Neutrophil migration from blood to tissue-residing microbes is governed by a series of chemoattractant gradients of both endogenous and microbial origin. Periodontal disease is characterized by neutrophil accumulation in the gingival pocket, recruited by the subgingival biofilm consisting mainly of gram-negative, anaerobic and proteolytic species such as . The fact that neutrophils are the dominating cell type in the gingival pocket suggests that neutrophil-specific chemoattractants are released by subgingival bacteria, but characterization of chemoattractants released by subgingival biofilm species remains incomplete. In the present study we characterized small (< 3 kDa) soluble chemoattractants released by growing , and show that these are selective for neutrophils. Most neutrophil chemoattractant receptors are expressed also by mononuclear phagocytes, the free fatty acid receptor 2 (FFAR2) being an exception. In agreement with the selective neutrophil recruitment, the chemotactic activity found in supernatants was mediated in part by a mixture of short chain fatty acids (SCFAs) that are recognized by FFAR2, and other leukocytes (including monocytes) did not respond to SCFA stimulation. Although SCFAs, produced by bacterial fermentation of dietary fiber in the gut, has previously been shown to utilize FFAR2, our data demonstrate that the pronounced proteolytic metabolism employed by (and likely also other subgingival biofilm bacteria associated with periodontal diseases) may result in the generation of SCFAs that attract neutrophils to the gingival pocket. This finding highlights the interaction between SCFAs and FFAR2 in the context of colonization during periodontal disease, but may also have implications for other inflammatory pathologies involving proteolytic bacteria.