Circular HDAC9/microRNA-138/Sirtuin-1 Pathway Mediates Synaptic and Amyloid Precursor Protein Processing Deficits in Alzheimer’s Disease

• Published in: Neuroscience bulletin Vol. 35; no. 5; pp. 877 - 888
• Main Authors: Lu, Yanjun, Tan, Lu, Wang, Xiong
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.10.2019; Springer
• Subjects: Advertising executives; Aged; Aged, 80 and over; Alzheimer Disease - genetics; Alzheimer Disease - metabolism; Alzheimer Disease - pathology; Alzheimer's disease; Amyloid beta-protein; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - metabolism; Anatomy; Anesthesiology; Animals; Biomedical and Life Sciences; Biomedicine; Female; Hippocampus - metabolism; Hippocampus - pathology; Histone Deacetylases - genetics; Histone Deacetylases - metabolism; Human Physiology; Humans; Male; Mice; Mice, Transgenic; MicroRNA; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Neurology; Neurosciences; Original; Original Article; Pain Medicine; Repressor Proteins - genetics; Repressor Proteins - metabolism; RNA, Circular - genetics; RNA, Circular - metabolism; Sirtuin 1 - genetics; Sirtuin 1 - metabolism; Synapses - genetics; Synapses - metabolism; Synapses - pathology; Sirtuin-1; microRNA; Circular RNA; Synapse; Alzheimer’s disease; Memory
• ISSN: 1673-7067; 1995-8218; 1995-8218
• DOI: 10.1007/s12264-019-00361-0

Synaptic dysfunction and abnormal processing of amyloid precursor protein (APP) are early pathological features in Alzheimer’s disease (AD). Recently, non-coding RNAs such as microRNAs (miRNAs) and circular RNAs (circRNAs) have been reported to contribute to the pathogenesis of AD. We found an age-dependent elevation of miR-138 in APP/PS1 (presenilin-1) mice. MiR-138 inhibited the expression of ADAM10 [a disintegrin and metalloproteinase domain-containing protein 10], promoted amyloid beta (Aβ) production, and induced synaptic and learning/memory deficits in APP/PS1 mice, while its suppression alleviated the AD-like phenotype in these mice. Overexpression of sirtuin 1 (Sirt1), a target of miR-138, ameliorated the miR-138-induced inhibition of ADAM10 and elevation of Aβ in vitro . The circRNA HDAC9 (circHDAC9) was predicted to contain a miR-138 binding site in several databases. Its expression was inversely correlated with miR-138 in both Aβ-oligomer-treated N2a cells and APP/PS1 mice, and it co-localized with miR-138 in the cytoplasm of N2a cells. CircHDAC9 acted as a miR-138 sponge, decreasing miR-138 expression, and reversing the Sirt1 suppression and excessive Aβ production induced by miR-138 in vitro . Moreover, circHDAC9 was decreased in the serum of both AD patients and individuals with mild cognitive impairment. These results suggest that the circHDAC9/miR-138/Sirt1 pathway mediates synaptic function and APP processing in AD, providing a potential therapeutic target for its treatment.