Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies

• Published in: Sensors (Basel, Switzerland) Vol. 19; no. 1; p. 52
• Main Authors: Jeong, Hee-Jin, Dong, Jinhua, Ueda, Hiroshi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.12.2018; MDPI
• Subjects: Antibodies, Anti-Idiotypic - immunology; Antigens; Binding sites; Communication; Epidemics; Fluorescence; H1N1; hemagglutinin; Hemagglutinin Glycoproteins, Influenza Virus - chemistry; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Hemagglutinin Glycoproteins, Influenza Virus - isolation & purification; Humans; Immunoglobulin Fab Fragments - chemistry; Immunoglobulin Fab Fragments - immunology; immunosensor; influenza A; Influenza A Virus, H1N1 Subtype - chemistry; Influenza A Virus, H1N1 Subtype - isolation & purification; Influenza A Virus, H1N1 Subtype - pathogenicity; Influenza, Human - diagnosis; Influenza, Human - immunology; Influenza, Human - virology; Labeling; Methods; Pandemics; Proteins; Quenchbody; Swine flu; Viruses; United States > US; Japan; immunosensor; influenza A; Quenchbody; H1N1; hemagglutinin
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s19010052

We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.