Bovine milk-derived exosomes for drug delivery

• Published in: Cancer letters Vol. 371; no. 1; pp. 48 - 61
• Main Authors: Munagala, Radha, Aqil, Farrukh, Jeyabalan, Jeyaprakash, Gupta, Ramesh C
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.02.2016; Elsevier Limited
• Subjects: Administration, Oral; Animals; Anticarcinogenic Agents - administration & dosage; Anticarcinogenic Agents - pharmacokinetics; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - pharmacokinetics; Automation; Brownian motion; Cattle; Cell Line, Tumor; Cell Proliferation - drug effects; Chemistry, Pharmaceutical; Chemopreventive agents; Chemotherapeutic drugs; Drug Carriers; Drug delivery; Exosomes - immunology; Exosomes - metabolism; Female; Folic Acid - metabolism; Hematology; Hematology, Oncology and Palliative Medicine; Humans; Immune Tolerance; Ligands; Lung Neoplasms - drug therapy; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Male; Mice, Nude; Milk; Milk - immunology; Milk - metabolism; Milk - toxicity; Milk-derived exosomes; Nanoparticles; Proteins; Rodents; Software; Solubility; Statistical analysis; Tissue Distribution; Tumor-targeting; Xenograft Model Antitumor Assays; Atomic force microscopy; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; AFM; Drug delivery; Anthos; Anthocyanidins; MTT; LPS; EGFR; Epidermal growth factor receptor; WFA; EMSA; Ultra-performance liquid chromatography; Paclitaxel; Lipopolysaccharide; miRNA; DOC; cDNA; Curcumin; folic acid; Vascular endothelial growth factor; PBS; Quantitative reverse transcription-polymerase chain reaction; Chemopreventive agents; Phosphate-buffered saline; Scanning electron microscopy; CUR; Withaferin A; Tumor-targeting; Chemotherapeutic drugs; VEGF; qRT-PCR; Docetaxel; Tumor necrosis factor-α; Milk-derived exosomes; Multivesicular bodies; PAC; TNF-α; UPLC; Complementary deoxynucleic acid; MVB; microRNA; SEM; Electrophoretic mobility shift assay (EMSA); FA
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2015.10.020

Highlights • Bovine milk is a biocompatible and scalable source of exosomes. • Milk exosomes can serve as a vehicle to deliver both hydrophilic and lipophilic small drug molecules. • Exosomal formulations provide enhanced biological efficacy. • Milk exosomes can be functionalized with ligands such as folic acid to achieve tumor targeting. • Milk exosomes per se exhibit protective effects presumably due to the endogenous cargo of macromolecules.