Phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumption

• Published in: Journal of mass spectrometry and advances in the clinical lab Vol. 22; pp. 3 - 7
• Main Authors: Neumann, Jasna, Beck, Olof, Böttcher, Michael
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2021; Elsevier
• Subjects: Alcohol biomarkers; Blood; Ethanol; Ethyl glucuronide; Liquid chromatography–mass spectrometry; Medicin och hälsovetenskap; Phosphatidylethanol; Ethanol; Ethyl glucuronide; EtG; Alcohol biomarkers; WB; Liquid chromatography–mass spectrometry; PEtH; LC-MSMS; Blood; Phosphatidylethanol; WB, whole blood; EtG, ethyl glucuronide; LC-MSMS, liquid chromatography tandem mass spectrometry; PEtH, phosphatidylethanol
• ISSN: 2667-145X; 2667-1468; 2667-145X
• DOI: 10.1016/j.jmsacl.2021.09.005

•PEth, EtG and ethanol were measured in 1149 clinical blood samples.•EtG and PEth concentrations seemed to be independent.•Their combined measurement in the same blood sample is possible.•This provides valuable information regarding recent alcohol consumption complementary to self-report. Alcohol biomarkers can monitor both recent and long-term drinking and provide information about drinking habits as a complement to self-reporting. Ethyl glucuronide (EtG) and phosphatidylethanol (PEth) are the most sensitive available biomarkers for this purpose. The present study aimed to collect data on both PEth and EtG in the same blood sample, in addition to ethanol, in order to evaluate the combined use of these biomarkers. Venous EDTA blood samples (n = 1149) sent to the laboratory as part of a clinical routine service for measuring PEth were investigated. PEth and EtG concentrations were analyzed using liquid chromatography–mass spectrometry methods and ethanol with an enzymatic method. Of the 1149 samples, 95 were positive for ethanol (range 0.11–3.12 g/L), 454 for EtG (1.0–9739 ng/mL), 635 for PEth (0.014–6.0 µmol/L), 534 for PEth ≥ 0.050 µmol/L, and 315 for PEth ≥ 0.30 µmol/L. EtG and PEth concentrations seemed largely independent as the coefficient of determination (r2) between PEth and EtG concentrations was 0.15. However, when the EtG concentrations were evaluated for different subgroups depending on ethanol or PEth concentrations a statistically significant difference between successive higher concentrations was observed. EtG and PEth are independent measures of recent alcohol drinking reflecting different time windows. Their combined measurement in the same blood sample is possible and will provide valuable information regarding recent alcohol consumption as a complement to self-reporting.