VAMP-1, VAMP-2, and syntaxin-4 regulate ANP release from cardiac myocytes

Abstract ANP is a peptide released by cardiac myocytes that regulates blood pressure and natriuresis. However, the molecular mechanisms controlling ANP release from cardiac myocytes are not defined. We now identify three components of the exocytic machinery that regulate ANP release from atrial myoc...

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Published inJournal of molecular and cellular cardiology Vol. 49; no. 5; pp. 791 - 800
Main Authors Ferlito, Marcella, Fulton, William B, Zauher, Mohamed A, Marbán, Eduardo, Steenbergen, Charles, Lowenstein, Charles J
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.11.2010
Subjects
Animals
Animals, Newborn
Atrial Natriuretic Factor - metabolism
Cardiovascular
Cell Separation
Endothelins - metabolism
Exocytosis
Granule
HeLa Cells
Humans
Hypertension
Mice
Myocytes, Cardiac - metabolism
N-ethylmaleimide sensitive factor
Nitric oxide
Protein Binding
Protein Transport
Qa-SNARE Proteins - metabolism
Rats
Secretion
Trafficking
Vesicle-Associated Membrane Protein 1 - metabolism
Vesicle-Associated Membrane Protein 2 - metabolism
Hypertension
N-ethylmaleimide sensitive factor
Secretion
Trafficking
Nitric oxide
Granule
Exocytosis
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Summary:Abstract ANP is a peptide released by cardiac myocytes that regulates blood pressure and natriuresis. However, the molecular mechanisms controlling ANP release from cardiac myocytes are not defined. We now identify three components of the exocytic machinery that regulate ANP release from atrial myocytes. We found that cardiac myocytes express N -ethylmaleimide sensitive factor (NSF), soluble NSF attachment protein (α-SNAP), and SNAP receptors (SNAREs). Additionally we found that specific SNARE molecules, VAMP-1 and VAMP-2, both co-sediment and co-localize with ANP. Also, one SNARE molecule, syntaxin-4, partially co-sediments and partially co-localizes with ANP. Furthermore, these three SNAREs, sytntaxin-4 and VAMP-1 and VAMP-2, form a SNARE complex inside cardiac myocytes. Finally, knockdown of VAMP-1, VAMP-2, or syntaxin-4 blocks regulated release of ANP. In contrast, silencing of VAMP-3 did not have an effect on ANP release. Our data suggest that three specific SNAREs regulate cardiac myocyte exocytosis of ANP. Pathways that modify the exocytic machinery may influence natriuresis and blood pressure.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-2828
1095-8584
DOI:10.1016/j.yjmcc.2010.08.020