Hepatitis E virus sequences in swine related to sequences in humans, The Netherlands

Hepatitis E virus (HEV), a major cause of viral hepatitis in much of the developing world, has recently been detected in swine in North America and Asia, raising concern about potential for zoonotic transmission. To investigate if HEV is commonly present in swine in the Netherlands, pooled stool sam...

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Published inEmerging infectious diseases Vol. 7; no. 6; pp. 970 - 976
Main Authors van der Poel, W H, Verschoor, F, van der Heide, R, Herrera, M I, Vivo, A, Kooreman, M, de Roda Husman, A M
Format Journal Article
LanguageEnglish
Published United States U.S. National Center for Infectious Diseases 01.11.2001
Centers for Disease Control and Prevention
Subjects
Animals
Cloning, Molecular
Diseases
Evaluation
Genetic aspects
Genetic vectors
Hepatitis E
Hepatitis E - epidemiology
Hepatitis E - veterinary
Hepatitis E - virology
Hepatitis E virus - classification
Hepatitis E virus - genetics
Hepatitis E virus - isolation & purification
Hepatitus E
HEV
Humans
Netherlands - epidemiology
Phylogeny
RNA, Viral - analysis
Swine
Swine Diseases - epidemiology
Swine Diseases - virology
the Netherlands
zoonoses
Netherlands
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Summary:Hepatitis E virus (HEV), a major cause of viral hepatitis in much of the developing world, has recently been detected in swine in North America and Asia, raising concern about potential for zoonotic transmission. To investigate if HEV is commonly present in swine in the Netherlands, pooled stool samples from 115 swine farms and nine individual pigs with diarrhea were assayed by reverse transcription-polymerase chain reaction (RT-PCR) amplification. HEV RNA was detected by RT-PCR and hybridization in 25 (22%) of the pooled specimens, but in none of the individual samples. RT-PCR amplification products of open reading frames 1 and 2 were sequenced, and the results were compared with published sequences of HEV genotypes from humans and swine. HEV strains from swine in the Netherlands were clustered in at least two groups, together with European and American isolates from swine and humans. Our data show that HEV in swine in the Netherlands are genetically closely related to HEV isolates from humans. Although zoonotic transmission has not been proven, these findings suggest that swine may be reservoir hosts of HEV.
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ISSN:1080-6040
1080-6059
DOI:10.3201/eid0706.010608