Downregulation of ICAM-1 and VCAM-1 expression in endothelial cells treated by photodynamic therapy

• Published in: Oncogene Vol. 23; no. 53; pp. 8649 - 8658
• Main Authors: VOLANTI, Cédric, GLOIRE, Geoffrey, VANDERPLASSCHEN, Alain, JACOBS, Nathalie, HABRAKEN, Yvette, PIETTE, Jacques
• Format: Journal Article Web Resource
• Language: English
• Published: Basingstoke Nature Publishing 11.11.2004; Nature Publishing Group
• Subjects: adhesion molecules; Apoptosis; Biochemistry, biophysics & molecular biology; Biochimie, biophysique & biologie moléculaire; Biological and medical sciences; Calpain; Cancer; Cell adhesion & migration; Cell Adhesion - drug effects; Cell adhesion molecules; Cell death; Cell Line; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Down-Regulation - drug effects; Endothelial cells; Endothelial Cells - drug effects; Fundamental and applied biological sciences. Psychology; Genes; Human health sciences; Humans; Immunology; Inflammation - genetics; Intercellular adhesion molecule 1; Intercellular Adhesion Molecule-1 - genetics; Intercellular Adhesion Molecule-1 - metabolism; Interleukin 6; Interleukin-6 - genetics; Interleukin-6 - metabolism; Intracellular Membranes - drug effects; Intracellular Membranes - metabolism; Leukocytes (granulocytic); Life sciences; Lysosomes - metabolism; Molecular and cellular biology; Neutrophils; Neutrophils - cytology; Neutrophils - drug effects; NF-kappa B; NF-kappa B - metabolism; NF-κB protein; Oncologie; Oncology; Oxidation-Reduction - drug effects; Oxidative stress; Pathology; Photochemotherapy; Photodynamic therapy; Photosensitization; Porphyrins - pharmacology; Proteasomes; Protein Binding; pyropheophorbide; Radiation; Reactive oxygen species; Sciences de la santé humaine; Sciences du vivant; Transcription; Transcription, Genetic - drug effects; Vascular cell adhesion molecule 1; Vascular Cell Adhesion Molecule-1 - genetics; Vascular Cell Adhesion Molecule-1 - metabolism; Belgium; Oxidative stress; pyropheophorbide; Endothelial cell; endothelial cells; Photodynamic therapy; adhesion molecules; NF-KB; Gene expression; Adhesion; Carcinogenesis
• ISSN: 0950-9232; 1476-5594; 1476-5594
• DOI: 10.1038/sj.onc.1207871

Photodynamic therapy (PDT) is a treatment for cancer and several noncancerous proliferating cell diseases that depends on the uptake of a photosensitizing compound followed by selective irradiation with visible light. In the presence of oxygen, irradiation leads to the production of reactive oxygen species (ROS). A large production of ROS induces the death of cancer cells by apoptosis or necrosis. A small ROS production can activate various cellular pathways. Here, we show that PDT by pyropheophorbide-a methyl ester (PPME) induces the activation of nuclear factor kappa B (NF-kappaB) in HMEC-1 cells. NF-kappaB is active since it binds to the NF-kappaB sites of both ICAM-1 and vascular cell adhesion molecule-1 (VCAM-1) promoters and induces the transcription of several NF-kappaB target genes such as those of IL-6, ICAM-1, VCAM-1. In contrast, expression of ICAM-1 and VCAM-1 at the protein level was not observed, although we measured an IL-6 secretion. Using specific chemical inhibitors, we showed that the lack of ICAM-1 and VCAM-1 expression is the consequence of their degradation by lysosomal proteases. The proteasome and calpain pathways were not involved. All these observations were consistent with the fact that no adhesion of granulocytes was observed in these conditions.