Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor

• Published in: Nucleic acids research Vol. 13; no. 16; pp. 5869 - 5882
• Main Authors: Furutani, Yasuji, Notake, Mitsue, Yamayoshi, Michiko, Yamagishi, Jun-ichi, Nomura, Hideki, Ohue, Mayumi, Furuta, Ryuji, Fukui, Toshikazu, Yamada, Masaaki, Nakamura, Shinichi
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 26.08.1985
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; cDNA; Cloning, Molecular; DNA - isolation & purification; DNA Restriction Enzymes; Escherichia coli; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Genes. Genome; Humans; hybridization analysis; interleukin 1; Interleukin-1 - genetics; Macrophages - metabolism; Molecular and cellular biology; Molecular genetics; Nucleic Acid Hybridization; nucleotide sequence; Plasmids; Protein Precursors - genetics; Rabbits; RNA, Messenger - genetics; Species Specificity; Human; Rabbit; Primary structure; Lagomorpha; Gene expression; Precursor; Vertebrata; Mammalia; Messenger RNA; Complementary DNA; Gene; Interleukin 1; Molecular cloning; Comparative study
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/13.16.5869

DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino add residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.