Cytokine-cytokine receptor interactions in the highly pathogenic avian influenza H5N1 virus-infected lungs of genetically disparate Ri chicken lines

• Published in: Animal bioscience Vol. 35; no. 3; pp. 367 - 376
• Main Authors: Vu, Thi Hao, Hong, Yeojin, Truong, Anh Duc, Lee, Jiae, Lee, Sooyeon, Song, Ki-Duk, Cha, Jihye, Dang, Hoang Vu, Tran, Ha Thi Thanh, Lillehoj, Hyun S, Hong, Yeong Ho
• Format: Journal Article
• Language: English
• Published: Korea (South) Asian - Australasian Association of Animal Production Societies 01.03.2022; Animal Bioscience; Asian-Australasian Association of Animal Production Societies; 아세아·태평양축산학회
• Subjects: Cell receptors; cytokine-cytokine receptor; Cytokines; Diseases; h5n1; Health aspects; highly pathogenic avian influenza virus (hpaiv); Influenza viruses; Physiological aspects; Poultry; ri chickens; rna sequencing; 축산학; Vietnam; Ri Chickens; Highly Pathogenic Avian Influenza Virus (HPAIV); H5N1; RNA Sequencing; Cytokine-cytokine Receptor
• ISSN: 2765-0189; 2765-0235; 2765-0235
• DOI: 10.5713/ab.21.0163

The highly pathogenic avian influenza virus (HPAIV) is a threat to the poultry industry as well as the economy and remains a potential source of pandemic infection in humans. Antiviral genes are considered a potential factor for HPAIV resistance. Therefore, in this study, we investigated gene expression related to cytokine-cytokine receptor interactions by comparing resistant and susceptible Ri chicken lines for avian influenza virus infection. Ri chickens of resistant (Mx/A; BF2/B21) and susceptible (Mx/G; BF2/B13) lines were selected by genotyping the Mx dynamin like GTPase (Mx) and major histocompatibility complex class I antigen BF2 genes. These chickens were then infected with influenza A virus subtype H5N1, and their lung tissues were collected for RNA sequencing. In total, 972 differentially expressed genes (DEGs) were observed between resistant and susceptible Ri chickens, according to the gene ontology and Kyoto encyclopedia of genes and genomes pathways. In particular, DEGs associated with cytokine-cytokine receptor interactions were most abundant. The expression levels of cytokines (interleukin-1β [IL-1β], IL-6, IL-8, and IL-18), chemokines (C-C Motif chemokine ligand 4 [CCL4] and CCL17), interferons (IFN-γ), and IFN-stimulated genes (Mx1, CCL19, 2'-5'-oligoadenylate synthaselike, and protein kinase R) were higher in H5N1-resistant chickens than in H5N1-susceptible chickens. Resistant chickens show stronger immune responses and antiviral activity (cytokines, chemokines, and IFN-stimulated genes) than those of susceptible chickens against HPAIV infection.