A novel immunoregulatory protein in human colostrum, syntenin-1, for promoting the development of IgA-producing cells from cord blood B cells

• Published in: International immunology Vol. 21; no. 9; pp. 1013 - 1023
• Main Authors: Sira, Mostafa M., Yoshida, Taketoshi, Takeuchi, Makoto, Kashiwayama, Yoshinori, Futatani, Takeshi, Kanegane, Hirokazu, Sasahara, Akiko, Ito, Yasunori, Mizuguchi, Mineyuki, Imanaka, Tsuneo, Miyawaki, Toshio
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.09.2009; Oxford Publishing Limited (England)
• Subjects: Animals; B cells; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; cell differentiation; Colostrum - immunology; cytokines; Female; Fetal Blood - immunology; Humans; Immunoglobulin A - biosynthesis; Immunoglobulin A - immunology; L Cells (Cell Line); Mice; Pregnancy; Syntenins - immunology; cell differentiation; cytokines; B cells
• ISSN: 0953-8178; 1460-2377
• DOI: 10.1093/intimm/dxp067

Human colostrum contains many bioactive factors that must promote the development of intestinal mucosal immunity in infants. Especially, the presence of certain cytokines such as transforming growth factor (TGF)-β or IL-10 has been of great interest for IgA production as a function of mucosal immune response. In the present study, we attempted to investigate whether unidentified factors inducing generation of IgA-producing cells from naive B cells might exist in colostrum. For this purpose, colostrum samples were directly added to a culture consisting of naive B cells and dendritic cells from cord blood and CD40 ligand-transfected L cells, comparing with recombinant IL-10 (rIL-10) and/or rTGF-β. It was noted that most colostrum samples alone were able to induce IgA-secreting cells at higher levels than rIL-10 and/or rTGF-β. IgA-inducing activity of colostrum was abolished by neither anti-neutralizing mAbs against IL-10 nor TGF-β, though partially by anti-IL-6 mAb. We prepared partially purified fractions from both pooled colostrums with and without IgA-inducing activity and comparatively performed quantitative proteomic analysis by two-dimensional difference gel electrophoresis followed by liquid chromatography-mass spectrometry. As a result, syntenin-1 was identified as a candidate for IgA-inducing protein in colostrum. Western blot analysis indicated that levels of syntenin-1 in colostrum samples were correlated with their IgA-inducing activities. Moreover, we demonstrated that recombinant syntenin-1 could induce preferentially IgA production from naive B cells. These results suggest that syntenin-1 serves as one of IgA-inducing factors for B cells.