Las21 participates in extracellular/cell surface phenomena in Saccharomyces cerevisiae

• Published in: Genes & Genetic Systems Vol. 74; no. 5; pp. 241 - 256
• Main Authors: Tohe, A. (Tokyo Univ. (Japan)), Oguchi, T
• Format: Journal Article
• Language: English
• Published: Japan The Genetics Society of Japan 01.10.1999; Japan Science and Technology Agency
• Subjects: Benzenesulfonates - pharmacology; calcofluor; CELLS; CELLULE; CELULAS; Fungal Proteins - genetics; Fungal Proteins - physiology; Gene Expression Regulation, Fungal; Glycosylphosphatidylinositols - physiology; Hydrolases - metabolism; INTERFACE; INTERFACE PHENOMENA; LAS21 gene; las21 protein; Magnesium - pharmacology; Membrane Proteins - genetics; Membrane Proteins - physiology; Mutation; PHENOMENE D'INTERFACE; Phenotype; PROTEINAS UNICELULARES; PROTEINE MICROBIOLOGIQUE; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - physiology; SINGLE CELL PROTEIN; Spores, Fungal; tetracaine
• ISSN: 1341-7568; 1880-5779
• DOI: 10.1266/ggs.74.241

Las21 (Yjl062W) is a member of the major facilitator super family, possessing multimembrane spanning domains. The LAS21 gene was identified as a responsible gene for a Saccharomyces cerevisiae mutan which shows sensitivity to a local anestheticum, tetracaine. The null las21 mutant (las21Δ) is viable but shows temperature sensitive growth. We found, in addition to this phenotype, that the las21Δ strain shows a number of defects; mating deficiency, calcofluor resistance, and formation of Zymolyase sensitive spores. Temperature sensitive growth of the las21Δ mutant was found to be suppressed by 0.1 M MgSO4. Two multicopy suppressors were obtained. They are ECM33 (YBR078W) and PIR2/HSP150 (YJR159W) both have some roles in an extracellular function. The common features of the suppressors, genetic and physiological, of the las21 Δ mutation suggest that Las21 participates in a global activity of extracellular phenomena. The las 21 phenotypes are consistent with the idea that Las21/Gpi7 acts in metabolism of glycosylphosphatidylinositol.