Progress of cationic gene delivery reagents for non-viral vector

• Published in: Applied microbiology and biotechnology Vol. 105; no. 2; pp. 525 - 538
• Main Authors: Ma, Kai, Mi, Chun-Liu, Cao, Xiang-Xiang, Wang, Tian-Yun
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 2021; Springer; Springer Nature B.V
• Subjects: Amino acids; Ammonium; Ammonium salts; Animals; Biomedical and Life Sciences; Biotechnology; Cationic polymerization; Cations; Chemical tests and reagents; Clinical trials; Equipment and supplies; Forecasts and trends; Gene therapy; Gene transfer; Gene Transfer Techniques; Genetic aspects; Genetic engineering; Genetic Therapy; Guanidine; Immunogenicity; Indicators and Reagents; Life Sciences; Liposomes; Methods; Microbial Genetics and Genomics; Microbiology; Mini-Review; Optimization; Polymers; Polypeptides; Proteins; Quaternary ammonium salts; Reagents; Transfection; Vectors (Biology); China; Liposome; Gene delivery; Transient expression system; Transfection optimization; Transfection reagent
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-020-11028-6

Gene delivery systems play a vital role in gene therapy and recombinant protein production. The advantages of using gene delivery reagents for non-viral vector include the capacity to accommodate a large packaging load and their low or absent immunogenicity. Furthermore, they are easy to produce at a large scale and preserve. Gene delivery reagents for non-viral vector are commonly used for transfecting a variety of cells and tissues. It is mainly composed of liposomes and non-liposome cationic polymers. According to the different head structures used, the non-viral cationic transfection reagents include a quaternary ammonium salt, amine, amino acid or polypeptide, guanidine salt, and a heterocyclic ring. This article summarizes these approaches and developments of types and components of transfection reagents and optimization of gene delivery. The optimization of mammalian cell transient recombinant protein expression system and cationic reagents for clinical or clinical trials are also discussed.