Quantitative imaging of selenoprotein with multi-isotope imaging mass spectrometry (MIMS)
Multi‐isotope imaging mass spectrometry (MIMS) allows high‐resolution quantitative imaging of protein and nucleic acid synthesis at the level of a single cell using stable isotope labels. We employed MIMS to determine the compartmental localization of selenoproteins tagged with stable isotope seleni...
Saved in:
Published in | Surface and interface analysis Vol. 46; no. S1; pp. 154 - 157 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Blackwell Publishing Ltd
01.11.2014
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Multi‐isotope imaging mass spectrometry (MIMS) allows high‐resolution quantitative imaging of protein and nucleic acid synthesis at the level of a single cell using stable isotope labels. We employed MIMS to determine the compartmental localization of selenoproteins tagged with stable isotope selenium compounds in human aortic endothelial cells (HAEC), and to compare the efficiency of labeling (to determine the ideal selenium source) from these compounds: [82Se]‐selenite, [77Se]‐seleno‐methionine, and [76Se]‐methyl‐selenocysteine. We found that all three selenium isotopes appear to be localized in the nucleus as well as in the cytoplasm. For MIMS detection, we compared freeze‐drying to thin layer versus thin sectioning for sample preparation. MIMS provides a unique and novel way to dissect selenoprotein synthesis in cells. Copyright © 2014 John Wiley & Sons, Ltd. |
---|---|
Bibliography: | istex:486406E1097B00540541AED231735ACE4B96EA75 Human Frontier Science Program - No. RGP0048 NIH - No. 5P41EB001974-13; No. AG034641; No. R01 AG040019; No. R21AG034641-01; No. R01 AG040209 ark:/67375/WNG-CCBDRBZ5-R Ellison Medical Foundation - No. AG-SS-2215-08; No. R37 HL61795; No. HL048743; No. HL108630 NIH ArticleID:SIA5625 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0142-2421 1096-9918 |
DOI: | 10.1002/sia.5625 |