RGS4蛋白在小儿肾母细胞瘤组织中的表达及临床意义
目的分析G蛋白信号调节蛋白4（RGS4）在小儿肾母细胞瘤组织和癌旁组织中的表达差异,探讨RGS4与小儿肾母细胞瘤发生发展的关系。方法收集37例小儿肾母细胞瘤患者术后肾癌组织及8例癌旁组织,采用免疫组织化学法检测RGS4蛋白的表达水平;另收集患者手术切除新鲜癌组织及对应癌旁组织标本各8例,采用q RT-PCR和Western blot分别检测RGS4 m RNA和蛋白的表达水平。结果免疫组织化学染色结果显示,RGS4蛋白在小儿肾母细胞瘤组织及癌旁组织中均存在阳性表达,其在小儿肾母细胞瘤组织中的高表达率低于癌旁组织[37.83%（14/37）vs.87.5%（7/8）,χ2=4.675,P〈0.0...
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| Published in | 天津医药 Vol. 45; no. 1; pp. 36 - 38 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
河北任丘华北石油管理局总医院儿科 邮编062552%河北承德市妇幼保健院新生儿科%河北任丘华北石油管理局总医院泌尿外科
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-9896 |
| DOI | 10.11958/20160888 |
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| Abstract | 目的分析G蛋白信号调节蛋白4（RGS4）在小儿肾母细胞瘤组织和癌旁组织中的表达差异,探讨RGS4与小儿肾母细胞瘤发生发展的关系。方法收集37例小儿肾母细胞瘤患者术后肾癌组织及8例癌旁组织,采用免疫组织化学法检测RGS4蛋白的表达水平;另收集患者手术切除新鲜癌组织及对应癌旁组织标本各8例,采用q RT-PCR和Western blot分别检测RGS4 m RNA和蛋白的表达水平。结果免疫组织化学染色结果显示,RGS4蛋白在小儿肾母细胞瘤组织及癌旁组织中均存在阳性表达,其在小儿肾母细胞瘤组织中的高表达率低于癌旁组织[37.83%（14/37）vs.87.5%（7/8）,χ2=4.675,P〈0.05];RGS4 m RNA在小儿肾母细胞瘤组织中的表达水平显著低于癌旁组织（1.064±0.549 vs.5.374±0.735,t=13.290,n=8,P〈0.01）;Western blot结果显示,RGS4蛋白在小儿肾母细胞瘤组织中的表达量低于癌旁组织（0.301±0.092 vs.0.779±0.041,t=13.424,n=8,P〈0.01）。结论 RGS4在小儿肾母细胞瘤组织中表达下调,可能与小儿肾癌的发生发展有关。 |
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| AbstractList | R737.11; 目的：分析G蛋白信号调节蛋白4（RGS4）在小儿肾母细胞瘤组织和癌旁组织中的表达差异，探讨RGS4与小儿肾母细胞瘤发生发展的关系。方法收集37例小儿肾母细胞瘤患者术后肾癌组织及8例癌旁组织，采用免疫组织化学法检测RGS4蛋白的表达水平；另收集患者手术切除新鲜癌组织及对应癌旁组织标本各8例，采用qRT-PCR和Western blot分别检测RGS4 mRNA和蛋白的表达水平。结果免疫组织化学染色结果显示，RGS4蛋白在小儿肾母细胞瘤组织及癌旁组织中均存在阳性表达，其在小儿肾母细胞瘤组织中的高表达率低于癌旁组织[37.83%（14/37）vs.87.5%（7/8），χ2=4.675，P<0.05]；RGS4 mRNA在小儿肾母细胞瘤组织中的表达水平显著低于癌旁组织（1.064±0.549 vs.5.374±0.735，t=13.290，n=8，P<0.01）；Western blot结果显示，RGS4蛋白在小儿肾母细胞瘤组织中的表达量低于癌旁组织（0.301±0.092 vs.0.779±0.041，t=13.424，n=8，P<0.01）。结论 RGS4在小儿肾母细胞瘤组织中表达下调，可能与小儿肾癌的发生发展有关。 目的分析G蛋白信号调节蛋白4（RGS4）在小儿肾母细胞瘤组织和癌旁组织中的表达差异,探讨RGS4与小儿肾母细胞瘤发生发展的关系。方法收集37例小儿肾母细胞瘤患者术后肾癌组织及8例癌旁组织,采用免疫组织化学法检测RGS4蛋白的表达水平;另收集患者手术切除新鲜癌组织及对应癌旁组织标本各8例,采用q RT-PCR和Western blot分别检测RGS4 m RNA和蛋白的表达水平。结果免疫组织化学染色结果显示,RGS4蛋白在小儿肾母细胞瘤组织及癌旁组织中均存在阳性表达,其在小儿肾母细胞瘤组织中的高表达率低于癌旁组织[37.83%（14/37）vs.87.5%（7/8）,χ2=4.675,P〈0.05];RGS4 m RNA在小儿肾母细胞瘤组织中的表达水平显著低于癌旁组织（1.064±0.549 vs.5.374±0.735,t=13.290,n=8,P〈0.01）;Western blot结果显示,RGS4蛋白在小儿肾母细胞瘤组织中的表达量低于癌旁组织（0.301±0.092 vs.0.779±0.041,t=13.424,n=8,P〈0.01）。结论 RGS4在小儿肾母细胞瘤组织中表达下调,可能与小儿肾癌的发生发展有关。 |
| Abstract_FL | Objective To investigate the expression levels of regulator of G-protein signaling 4 (RGS4) in pediatric nephroblastoma and pericancerous tissues, and explore the relationship between RGS4 and the occurrence and development of pediatric nephroblastoma. Methods Thirty-seven samples of pediatric nephroblastoma tissues and 8 samples of pericancerous tissues were collected after surgery to detect the expression of RGS4 protein by immunohistochemistry. Another 8 samples of fresh cancer tissues and corresponding pericancerous tissues were collected to detect the mRNA and protein levels of RGS4 by qRT-PCR and Western blot assay, respectively. Results Immunohistochemistry results showed that RGS4 protein was positively expressed both in pediatric nephroblastoma and pericancerous tissues, and its high expression rate was lower in pediatric nephroblastoma than that in pericancerous tissues [(37.83%(14/37) vs. 87.5%(7/8),χ2=4.675, P<0.05]. The expression level of RGS4 mRNA was significantly lower in pediatric nephroblastoma than that in pericancerous tissues (1.064 ± 0.549 vs. 5.374 ± 0.735, t=13.290, n=8, P < 0.01). Western blot results showed that the expression level of RGS4 protein was lower in pediatric nephroblastoma than that of pericancerous tissues (0.301±0.092 vs. 0.779 ± 0.041, t=13.424, n=8, P < 0.01). Conclusion The expression level of RGS4 is down-regulated in pediatric nephroblastoma, which may be related to the occurrence and development of pediatric nephroblastoma. |
| Author | 刘燕春 刘士霞 王晓静 王连芳 杨继宏 |
| AuthorAffiliation | 河北任丘华北石油管理局总医院儿科,062552 河北承德市妇幼保健院新生儿科 河北任丘华北石油管理局总医院泌尿外科 |
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| Author_FL | LIU Shi-xia WANG Lian-fang LIU Yan-chun WANG Xiao-jing YANG Ji-hong |
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| DocumentTitleAlternate | Clinical significance of expression of RGS4 in pediatric nephroblastoma tissue |
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| Keywords | 肾肿瘤 RGS4 pediatric nephroblastoma 基因表达 小儿肾母细胞瘤 RGS proteins kidney neoplasms RGS蛋白质类 G蛋白信号调节蛋白4 gene expression |
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| Notes | Objective To investigate the expression levels of regulator of G-protein signaling 4（RGS4） in pediatricnephroblastoma and pericancerous tissues, and explore the relationship between RGS4 and the occurrence and developmentof pediatric nephroblastoma. Methods Thirty- seven samples of pediatric nephroblastoma tissues and 8 samples ofpericancerous tissues were collected after surgery to detect the expression of RGS4 protein by immunohistochemistry.Another 8 samples of fresh cancer tissues and corresponding pericancerous tissues were collected to detect the m RNA andprotein levels of RGS4 by q RT-PCR and Western blot assay, respectively. Results Immunohistochemistry results showedthat RGS4 protein was positively expressed both in pediatric nephroblastoma and pericancerous tissues, and its highexpression rate was lower in pediatric nephroblastoma than that in pericancerous tissues [（37.83%（14/37） vs. 87.5%（7/8）,χ2=4.675, P〈0.05]. The expression level of RGS4 m RNA was significantly lower in pediatric nephroblastoma |
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| Publisher | 河北任丘华北石油管理局总医院儿科 邮编062552%河北承德市妇幼保健院新生儿科%河北任丘华北石油管理局总医院泌尿外科 |
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| Snippet | 目的分析G蛋白信号调节蛋白4（RGS4）在小儿肾母细胞瘤组织和癌旁组织中的表达差异,探讨RGS4与小儿肾母细胞瘤发生发展的关系。方法收集37例小儿肾母细胞瘤患者术后肾癌组织及8例癌旁组织,采用免疫组织化学法检测RGS4蛋白的表达水平;另收集患者手术切除新鲜癌组织及对应癌旁组织标本各8例,采用q... R737.11;... |
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| SubjectTerms | G蛋白信号调节蛋白4 RGS蛋白质类 基因表达 小儿肾母细胞瘤 肾肿瘤 |
| Title | RGS4蛋白在小儿肾母细胞瘤组织中的表达及临床意义 |
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