Neutrophil chemokines secreted by tumor cells mount a lung antimetastatic response during renal cell carcinoma progression

• Published in: Oncogene Vol. 32; no. 14; pp. 1752 - 1760
• Main Authors: López-Lago, M A, Posner, S, Thodima, V J, Molina, A M, Motzer, R J, Chaganti, R S K
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.04.2013; Nature Publishing Group
• Subjects: 631/250/2504/223/1699; 631/67/322; 631/67/580; 631/67/589/1588/1351; Adult; Animals; Apoptosis; Carcinoma, Renal cell; Carcinoma, Renal Cell - metabolism; Carcinoma, Renal Cell - pathology; Carcinoma, Renal Cell - prevention & control; Causes of; Cell Biology; Cell Line, Tumor; Cell Proliferation; Chemokines; Chemokines - antagonists & inhibitors; Chemokines - genetics; Chemokines - secretion; Cytotoxicity; Development and progression; Disease Progression; Fluorescent Antibody Technique; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Human Genetics; Humans; Interleukin 8; Internal Medicine; Kidney cancer; Kidney Neoplasms - metabolism; Kidney Neoplasms - pathology; Kidney Neoplasms - prevention & control; Leukocytes; Leukocytes (neutrophilic); Lung cancer; Lung carcinoma; Lung Neoplasms - metabolism; Lung Neoplasms - prevention & control; Lung Neoplasms - secondary; Male; Medicine; Medicine & Public Health; Metastases; Metastasis; Metastatic seeding; Mice; Mice, Inbred BALB C; Microenvironments; Neutrophils; Neutrophils - metabolism; Oncology; original-article; Real-Time Polymerase Chain Reaction; Renal cell carcinoma; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Tumor cell lines; Tumor cells; Tumors; United States; kidney cancer; metastasis; neutrophil
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/onc.2012.201

The mechanism by which renal cell carcinoma (RCC) colonizes the lung microenvironment during metastasis remains largely unknown. To investigate this process, we grafted human RCC cells with varying lung metastatic potential in mice. Gene expression profiling of the mouse lung stromal compartment revealed a signature enriched for neutrophil-specific functions that was induced preferentially by poorly metastatic cells. Analysis of the gene expression signatures of tumor cell lines showed an inverse correlation between metastatic activity and the levels of a number of chemokines, including CXCL5 and IL8 . Enforced depletion of CXCL5 and IL8 in these cell lines enabled us to establish a functional link between lung neutrophil infiltration, secretion of chemokines by cancer cells and metastatic activity. We further show that human neutrophils display a higher cytotoxic activity against poorly metastatic cells compared with highly metastatic cells. Together, these results support a model in which neutrophils recruited to the lung by tumor-secreted chemokines build an antimetastatic barrier with loss of neutrophil chemokines in tumor cells acting as a critical rate-limiting step during lung metastatic seeding.