Curcumin inhibits the side population (SP) phenotype of the rat C6 glioma cell line: Towards targeting of cancer stem cells with phytochemicals

• Published in: Cancer letters Vol. 293; no. 1; pp. 65 - 72
• Main Authors: Fong, Dunne, Yeh, Arthur, Naftalovich, Rotem, Choi, Theresa Hyejeong, Chan, Marion M
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.07.2010; Elsevier Limited
• Subjects: Animals; Antineoplastic Agents, Phytogenic - pharmacology; Benzimidazoles - pharmacokinetics; Breast cancer; C6 glioma; Cancer stem cells; Cell Differentiation - drug effects; Cell Growth Processes - drug effects; Cell Line, Tumor; Curcuma longa; Curcumin; Curcumin - pharmacology; Flow Cytometry; Fluorescent Dyes - pharmacokinetics; Glioma - drug therapy; Glioma - metabolism; Glioma - pathology; Glioma cells; Hematology, Oncology and Palliative Medicine; Herbal medicine; Inflammation; Medical research; Neoplastic Stem Cells - drug effects; Neoplastic Stem Cells - metabolism; Neoplastic Stem Cells - pathology; Phenotype; Rats; Side population; Spices; Stem cells; Verapamil - pharmacology; C6 glioma; Curcumin; Cancer stem cells; Side population
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2009.12.018

Abstract The phytochemical curcumin, from the Indian spice turmeric, has many biological properties, including anti-inflammatory and anti-carcinogenic activities. We have examined the effects of curcumin on the rat C6 glioma cell line. Treated and control cells were analyzed by Hoechst 33342 dye and flow cytometry. We observed a decrease in the side population (SP) of C6 cells after daily curcumin treatment of the C6 cells. Direct incubation of curcumin to C6 cells during the Hoechst assay also decreased SP. Since SP has been associated with stem cell populations, curcumin may be a dietary phytochemical with potential to target cancer stem cells.