Recombination Walking: Genetic Selection of Clones from Pooled Libraries of Yeast Artificial Chromosomes by Homologous Recombination

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 90; no. 17; pp. 8118 - 8122
• Main Authors: Miller, Allan M., Savinelli, Elizabeth A., Couture, Sandra M., Hannigan, Gene M., Han, Zhiyi, Selden, Richard F., Treco, Douglas A.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.09.1993; National Acad Sciences; National Academy of Sciences
• Subjects: 550400 - Genetics; Animals; BASIC BIOLOGICAL SCIENCES; Chromosome Mapping; Chromosome Walking; CHROMOSOMES; Chromosomes, Fungal; Disease; DISEASES; DNA; DNA probes; EUMYCOTA; FUNGI; GENE RECOMBINATION; Genes; Genes, Fungal; GENETIC MAPPING; Genetic screening; Genetics; Genomic Library; Genotype; Hominidae - genetics; Homologous recombination; HUMAN CHROMOSOMES; Human genetics; Humans; Libraries; MAPPING; Medical research; Medical screening; MICROORGANISMS; Mutation; PLANTS; Plasmids; Recombination, Genetic; Restriction Mapping; Saccharomyces cerevisiae - genetics; Selection, Genetic; Walking; YEASTS
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.90.17.8118

Recombination walking is based on the genetic selection of specific human clones from a yeast artificial chromosome (YAC) library by homologous recombination. The desired clone is selected from a pooled (unordered) YAC library, eliminating labor-intensive steps typically used in organizing and maintaining ordered YAC libraries. Recombination walking represents an efficient approach to library screening and is well suited for chromosome-walking approaches to the isolation of genes associated with common diseases.