Efficient Propagation of Equine Viruses in a Newly Established Equine Cell Line, FHK-Tcl3.1 Cells

Equine cells are required for isolation of viruses that infect the horse. However, only a few equine cell lines and cell cultures are available so far. Fetal horse kidney (FHK)-Tcl3.1 cell is a novel cell line established by introducing simian virus 40 (SV40) large T antigen. In the present study, t...

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Published inJournal of Veterinary Medical Science Vol. 75; no. 9; pp. 1223 - 1225
Main Authors OGUMA, Keisuke, ISHIDA, Maiko, MAEDA, Ken, SENTSUI, Hiroshi
Format Journal Article
LanguageEnglish
Published Tokyo JAPANESE SOCIETY OF VETERINARY SCIENCE 2013
Japan Science and Technology Agency
Subjects
cell line
equine virus
horse
viral disease
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Abstract Equine cells are required for isolation of viruses that infect the horse. However, only a few equine cell lines and cell cultures are available so far. Fetal horse kidney (FHK)-Tcl3.1 cell is a novel cell line established by introducing simian virus 40 (SV40) large T antigen. In the present study, the ability to propagate equine viruses was compared between FHK-Tcl3.1 cells and other equine cells. FHK-Tcl3.1 cells efficiently increased many viruses derived from or having pathogenicity to horses and produced high infective titers in culture fluids. These results indicate that FHK-Tcl3.1 cells would be useful for propagation and serological tests of viruses that affect Equidae.
AbstractList Equine cells are required for isolation of viruses that infect the horse. However, only a few equine cell lines and cell cultures are available so far. Fetal horse kidney (FHK)-Tcl3.1 cell is a novel cell line established by introducing simian virus 40 (SV40) large T antigen. In the present study, the ability to propagate equine viruses was compared between FHK-Tcl3.1 cells and other equine cells. FHK-Tcl3.1 cells efficiently increased many viruses derived from or having pathogenicity to horses and produced high infective titers in culture fluids. These results indicate that FHK-Tcl3.1 cells would be useful for propagation and serological tests of viruses that affect Equidae.
Author OGUMA, Keisuke
SENTSUI, Hiroshi
MAEDA, Ken
ISHIDA, Maiko
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  organization: Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252–0880, Japan
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  fullname: SENTSUI, Hiroshi
  organization: Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252–0880, Japan
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CitedBy_id crossref_primary_10_3390_vetsci12030228
crossref_primary_10_1007_s11626_016_0046_9
crossref_primary_10_1016_j_jviromet_2024_114952
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Cites_doi 10.1292/jvms.69.989
10.1128/JCM.38.12.4523-4526.2000
10.2460/ajvr.1974.35.09.1153
10.1294/jes.20.11
10.2222/jsv.18.214
10.1016/S0034-5288(18)32657-2
10.1111/j.1751-0813.1973.tb09291.x
10.1099/0022-1317-34-3-421
10.1292/jvms1939.39.117
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– reference: 1. Allen, G. P. and Bryans, J. T. 1974. Studies of an established equine cell line derived from a transitional cell carcinoma. Am. J. Vet. Res. 35: 1153–1160.
– reference: 5. Guy, J. S., Breslin, J. J., Breuhaus, B., Vivrette, S. and Smith, L. G. 2000. Characterization of a coronavirus isolated from a diarrheic foal. J. Clin. Microbiol. 38: 4523–4526.
– reference: 3. Andoh, K., Kai, K., Matsumura, T. and Maeda, K. 2009. Further development of an equine cell line that can be propagated over 100 times. J. Equine Sci. 20: 11–14.
– reference: 12. Nakano, K., Aida, Y., Seki, M., Sasaki, T. and Sasahara, J. 1981. Establishment of a cell line from fetal equine kidney. p. 149. In: Abstracts of the 91st Meeting of the Japanese Society of Veterinary Science. Tokyo (in Japanese).
– reference: 16. Wilks, C. R. and Studdert, M. J. 1973. The characterization of an equine adenovirus. Aust. Vet. J. 49: 456–459.
– reference: 6. Itoh, H., Morimoto, Y., Doi, Y., Sanpe, T. and Tsunoda, H. 1968. Viral susceptibility of an African green monkey kidney dell line-Vero. Virus 18: 10–24(in Japanese).
– reference: 11. Maeda, K., Yasumoto, S., Tsuruda, A., Andoh, K., Kai, K., Otoi, T. and Matsumura, T. 2007. Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses. J. Vet. Med. Sci. 69: 989–991.
– reference: 10. Kono, Y. and Kobayashi, K. 1964. Cytopathogenic equine orphan (CEO) virus in horse kidney cell culture. I. Isolation and properties. Natl. Inst. Anim. Health Q 4: 10–20.
– reference: 13. Oya, A., Sakumoto, H. and Kobayashi, M. 1963. Variation of virulence of Japanese B encephalitis virus to adult mice. Jpn. J. Med. Sci. Biol. 16: 50–52.
– reference: 14. Sentsui, H. and Kono, Y. 1980. An epidemic of Getah virus infection among racehorses in Japan: Isolation of the virus. Res. Vet. Sci. 29: 157–161.
– reference: 2. Allen, G. P. and Bryans, J. T. 1977. Replication of equine herpesvirus type 3: kinetics of infectious particle formation and virus nucleic acid synthesis. J. Gen. Virol. 34: 421–430.
– reference: 4. Doll, E. R., Bryans, J. T., McCollum, W. H. and Crowe, M. E. W. 1957. Isolation of filterable agent causing arteritis of horses and abortion by mares; its differentiation from the equine abortion (influenza) virus. Cornell Vet. 47: 3–41.
– reference: 15. Sugiura, T., Matsumura, T. and Fukunaga, Y. 1989. Isolation and identification of viruses from racehorses with pyrexia. Bull. Equine Res. Inst. 26: 53–59.
– reference: 7. Kawakami, Y., Kaji, T., Ishizaki, R., Shimizu, T. and Matsumoto, M. 1962. Etiologic study on an outbreak of acute respiratory disease among colts due to equine rhinopneumonitis virus. Jpn. J. Exp. Med. 32: 211–229.
– reference: 8. Kawakami, Y., Tokui, T., Nakano, T., Kume, K., Hiramune, T. and Murase, N. 1970. An outbreak of abortion due to equine rhinopneumonitis virus among mares in the Hidaka district, Hokkaido. I. Epizootiological survey and virus isolation. Bull. Natl. Inst. Anim. Health 61: 9–16(in Japanese).
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  doi: 10.2460/ajvr.1974.35.09.1153
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  doi: 10.1294/jes.20.11
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  doi: 10.2222/jsv.18.214
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  doi: 10.1016/S0034-5288(18)32657-2
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  doi: 10.1111/j.1751-0813.1973.tb09291.x
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SubjectTerms cell line
equine virus
horse
viral disease
Title Efficient Propagation of Equine Viruses in a Newly Established Equine Cell Line, FHK-Tcl3.1 Cells
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