Efficient Propagation of Equine Viruses in a Newly Established Equine Cell Line, FHK-Tcl3.1 Cells

Equine cells are required for isolation of viruses that infect the horse. However, only a few equine cell lines and cell cultures are available so far. Fetal horse kidney (FHK)-Tcl3.1 cell is a novel cell line established by introducing simian virus 40 (SV40) large T antigen. In the present study, t...

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Published inJournal of Veterinary Medical Science Vol. 75; no. 9; pp. 1223 - 1225
Main Authors OGUMA, Keisuke, ISHIDA, Maiko, MAEDA, Ken, SENTSUI, Hiroshi
Format Journal Article
LanguageEnglish
Published Tokyo JAPANESE SOCIETY OF VETERINARY SCIENCE 2013
Japan Science and Technology Agency
Subjects
cell line
equine virus
horse
viral disease
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Summary:Equine cells are required for isolation of viruses that infect the horse. However, only a few equine cell lines and cell cultures are available so far. Fetal horse kidney (FHK)-Tcl3.1 cell is a novel cell line established by introducing simian virus 40 (SV40) large T antigen. In the present study, the ability to propagate equine viruses was compared between FHK-Tcl3.1 cells and other equine cells. FHK-Tcl3.1 cells efficiently increased many viruses derived from or having pathogenicity to horses and produced high infective titers in culture fluids. These results indicate that FHK-Tcl3.1 cells would be useful for propagation and serological tests of viruses that affect Equidae.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 14
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.12-0450