Effect of Human Mesenchymal Stem Cells on the Growth of HepG2 and Hela Cells

• Published in: Cell Structure and Function Vol. 38; no. 1; pp. 109 - 121
• Main Authors: Long, Xiaohui, Matsumoto, Rena, Yang, Pengyuan, Uemura, Toshimasa
• Format: Journal Article
• Language: English
• Published: Japan Japan Society for Cell Biology 01.01.2013; Japan Science and Technology Agency
• Subjects: Cell Communication - drug effects; Cell Communication - physiology; Cell Proliferation - drug effects; Coculture Techniques; Culture Media, Conditioned - pharmacology; Female; Hela cells; HeLa Cells - drug effects; HeLa Cells - pathology; HeLa Cells - physiology; Hep G2 Cells - drug effects; Hep G2 Cells - pathology; Hep G2 Cells - physiology; HepG2 cells; human mesenchymal stem cells; Humans; internalizing quantum dots; Liver Neoplasms - pathology; Liver Neoplasms - physiopathology; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - physiology; Mitogen-Activated Protein Kinase Kinases - physiology; Phosphatidylinositol 3-Kinases - physiology; Signal Transduction - drug effects; Signal Transduction - physiology; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - physiopathology
• ISSN: 0386-7196; 1347-3700
• DOI: 10.1247/csf.12029

Human mesenchymal stem cells (hMSCs) accumulate at carcinomas and have a great impact on cancer cell’s behavior. Here we demonstrated that hMSCs could display both the promotional and inhibitive effects on growth of HepG2 and Hela cells by using the conditioned media, indirect co-culture, and cell-to-cell co-culture. Cell growth was increased following the addition of lower proportion of hMSCs while decreased by treatment of higher proportion of hMSCs. We also established a novel noninvasive label way by using internalizing quantum dots (i-QDs) for study of cell-cell contact in the co-culture, which was effective and sensitive for both tracking and distinguishing different cells population without the disturbance of cells. Furthermore, we investigated the role of hMSCs in regulation of cell growth and showed that mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) signaling pathways were involved in hMSC-mediated cell inhibition and proliferation. Our findings suggested that hMSCs regulated cancer cell function by providing a suitable environment, and the discovery from the study would provide some clues for development of effective strategy for hMSC-based cancer therapies.