Monitoring bisphenol A and its biodegradation in water using a fluorescent molecularly imprinted chemosensor

• Published in: Chemosphere (Oxford) Vol. 119; pp. 515 - 523
• Main Authors: Wu, Ya-ting, Liu, Yan-jie, Gao, Xia, Gao, Kai-chun, Xia, Hu, Luo, Mi-fang, Wang, Xue-juan, Ye, Lei, Shi, Yun, Lu, Bin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2015
• Subjects: Benzhydryl Compounds - analysis; Benzhydryl Compounds - metabolism; Biodegradation; Biodegradation, Environmental; Bisphenol A; Brackish; Chemoreceptors; Distilled water; Earth and Related Environmental Sciences; Environmental monitoring; Environmental Monitoring - methods; Environmental Sciences; Fluorescence; Fluorescent chemosensor; Fresh Water - chemistry; Geovetenskap och miljövetenskap; Imprinted polymers; Methacrylates - chemistry; Microplate reader; Miljövetenskap; Molecular Imprinting; Molecularly imprinted polymer; Monitors; Natural Sciences; Naturvetenskap; Phenols - analysis; Phenols - metabolism; Polymers - chemistry; Real time; Real-time monitoring; Water Pollutants, Chemical - analysis; Fluorescent chemosensor; fNIPs; HXT; TRIM; HBA; BPZ; E2; BP; Real-time monitoring; P; fMIPcs; T; MIPs; PNZ; Biodegradation; fMIPs; AIBN; Bisphenol A; BPA; BPC; TI; HAP; Molecularly imprinted polymer; Microplate reader; FTF; NIPs; Bold; IPB; Bmax; ELISA
• ISSN: 0045-6535; 1879-1298; 1879-1298
• DOI: 10.1016/j.chemosphere.2014.07.017

[Display omitted] •A new fluorescent MIP (fMIP) was prepared using dansyl methacrylate as monomer.•The first example of fMIP sensor (fMIPcs) by combining fMIP with microplate reader.•The fMIPcs quantified BPA in water and in organic solvent accurately.•For the first time, the fMIPcs allowed real-time monitoring of BPA biodegradation.•The analytical results obtained with the fMIPcs have been verified by HPLC analysis. In this paper, we present a simple and rapid method for monitoring bisphenol A (BPA) and its biodegradation in environmental water using a fluorescent molecularly imprinted polymer chemosensor (fMIPcs). A fluorescent molecularly imprinted polymer (fMIP) was first synthesized by precipitation polymerization method using BPA as template, dansyl methacrylate as functional monomer. Then a fMIPcs was constructed by combining the fMIP with a fluorescent microplate reader. The fMIPcs displayed selective, concentration-dependent fluorescence quenching in response to BPA in water even in the existence of interferences, thereby allowing reliable high through-put quantification of BPA via simple fluorescence measurements. The fMIPcs was able to directly quantify BPA (from 10 to 2000μgL−1) in different environmental water samples (distilled water, distilled water containing heavy metals and humic acid, tap water, and river water) with high accuracy, and to monitor BPA biodegradation in real-time. Using the fMIPcs, it was possible to achieve fast analytical results with lower limit of detection for BPA (3μgL−1) from smaller sample volume (250μL), which are superior to many relevant methods reported in the literature. Moreover, BPA levels and biodegradation rates measured by fMIPcs are comparable to the instrument-based method (HPLC). The fMIPcs developed in this work offers a new solution for simple, rapid, accurate and high through-put BPA quantification, and makes it possible to monitor BPA biodegradation in real time.