Physicochemical Characterization of the Strychnos Alkaloids by Capillary Zone Electrophoresis

A capillary zone electrophoresis (CZE) method has been developed for investigating the physicochemical characteristics of five Strychnos alkaloids in Strychnos nux-vomica L. Firstly, the dissociation constants of the five Strychnos alkaloids were determined, based on the relation between the effecti...

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Published inAnalytical Sciences Vol. 21; no. 11; pp. 1303 - 1308
Main Authors FU, Shaoping, ZHANG, Feng, ZHANG, Xiaozhe, XU, Qing, XIAO, Hongbin, LIANG, Xinmiao
Format Journal Article
LanguageEnglish
Published Japan The Japan Society for Analytical Chemistry 2005
Japan Science and Technology Agency
Subjects
Alkaloids - analysis
Alkaloids - chemistry
Alkaloids - isolation & purification
Electrophoresis, Capillary - methods
Hydrogen-Ion Concentration
Molecular Structure
Molecular Weight
Quinolizines
Sophora - chemistry
Strychnine - analogs & derivatives
Strychnine - chemistry
Strychnos nux-vomica - chemistry
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Summary:A capillary zone electrophoresis (CZE) method has been developed for investigating the physicochemical characteristics of five Strychnos alkaloids in Strychnos nux-vomica L. Firstly, the dissociation constants of the five Strychnos alkaloids were determined, based on the relation between the effective mobility of the solutes and the buffer pH. The mathematical relationship was strictly deduced from the fundamental electrophoretic theory and the dissociation equilibrium. Secondly, an equation describing the relation between the migration time of alkaloids of similar structure and their molecular weights was developed and used to predict the migration order and to calculate the electrosomotic velocity. The results predicted by the theory agreed with those from experiments.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.21.1303