Myocyte remodeling in response to hypertrophic stimuli requires nucleocytoplasmic shuttling of muscle LIM protein

• Published in: Journal of molecular and cellular cardiology Vol. 47; no. 4; pp. 426 - 435
• Main Authors: Boateng, Samuel Y, Senyo, Samuel E, Qi, Lixin, Goldspink, Paul H, Russell, Brenda
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2009
• Subjects: Amino Acid Sequence; Animals; Animals, Newborn; Cardiovascular; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Cells, Cultured; Hypertrophy; LIM Domain Proteins; Mechanosensing; Mechanotransduction; Molecular Sequence Data; Muscle Proteins - metabolism; Myocardial Contraction - drug effects; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; Nuclear Localization Signals - chemistry; Nuclear Localization Signals - metabolism; Nucleocytoplasmic shuttling; Peptides - chemistry; Peptides - pharmacology; Protein Transport - drug effects; Rats; Rats, Sprague-Dawley; Sarcomere remodeling; Stress, Mechanical; Subcellular Fractions - drug effects; Subcellular Fractions - metabolism; Transcription, Genetic - drug effects; Mechanotransduction; Mechanosensing; Nucleocytoplasmic shuttling; Sarcomere remodeling; Hypertrophy
• ISSN: 0022-2828; 1095-8584
• DOI: 10.1016/j.yjmcc.2009.04.006

Abstract CSRP3 or muscle LIM protein (MLP) is a nucleocytoplasmic shuttling protein and a mechanosensor in cardiac myocytes. MLP regulation and function was studied in cultured neonatal rat myocytes treated with pharmacological or mechanical stimuli. Either verapamil or BDM decreased nuclear MLP while phenylephrine and cyclic strain increased it. These results suggest that myocyte contractility regulates MLP subcellular localization. When RNA polymerase II was inhibited with α-amanitin, nuclear MLP was reduced by 30%. However, when both RNA polymerase I and II were inhibited with actinomycin D, there was a 90% decrease in nuclear MLP suggesting that its nuclear translocation is regulated by both nuclear and nucleolar transcriptional activity. Using cell permeable synthetic peptides containing the putative nuclear localization signal (NLS) of MLP, nuclear import of the protein in cultured rat neonatal myocytes was inhibited. The NLS of MLP also localizes to the nucleolus. Inhibition of nuclear translocation prevented the increased protein accumulation in response to phenylephrine. Furthermore, cyclic strain of myocytes after prior NLS treatment to remove nuclear MLP resulted in disarrayed sarcomeres. Increased protein synthesis and brain natriuretic peptide expression were also prevented suggesting that MLP is required for remodeling of the myofilaments and gene expression. These findings suggest that nucleocytoplasmic shuttling MLP plays an important role in the regulation of the myocyte remodeling and hypertrophy and is required for adaptation to hypertrophic stimuli.