Rotational manipulation of single cells and organisms using acoustic waves

The precise rotational manipulation of single cells or organisms is invaluable to many applications in biology, chemistry, physics and medicine. In this article, we describe an acoustic-based, on-chip manipulation method that can rotate single microparticles, cells and organisms. To achieve this, we...

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Published inNature communications Vol. 7; no. 1; p. 11085
Main Authors Ahmed, Daniel, Ozcelik, Adem, Bojanala, Nagagireesh, Nama, Nitesh, Upadhyay, Awani, Chen, Yuchao, Hanna-Rose, Wendy, Huang, Tony Jun
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 23.03.2016
Nature Publishing Group
Nature Portfolio
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Summary:The precise rotational manipulation of single cells or organisms is invaluable to many applications in biology, chemistry, physics and medicine. In this article, we describe an acoustic-based, on-chip manipulation method that can rotate single microparticles, cells and organisms. To achieve this, we trapped microbubbles within predefined sidewall microcavities inside a microchannel. In an acoustic field, trapped microbubbles were driven into oscillatory motion generating steady microvortices which were utilized to precisely rotate colloids, cells and entire organisms (that is, C. elegans ). We have tested the capabilities of our method by analysing reproductive system pathologies and nervous system morphology in C. elegans . Using our device, we revealed the underlying abnormal cell fusion causing defective vulval morphology in mutant worms. Our acoustofluidic rotational manipulation (ARM) technique is an easy-to-use, compact, and biocompatible method, permitting rotation regardless of optical, magnetic or electrical properties of the sample under investigation. The precise rotational manipulation of single cells is technically challenging and relies on the optical, magnetic and electrical properties of the biospecimen. Here the authors develop an acoustic-based, on-chip manipulation method that can rotate single microparticles, cells and organisms.
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These authors contributed equally to this work
ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms11085