Direct and consensual murine pupillary reflex metrics: Establishing normative values

• Published in: Autonomic neuroscience Vol. 151; no. 2; pp. 164 - 167
• Main Authors: Hussain, Rehana Z, Hopkins, Steven C, Frohman, Elliot M, Eagar, Todd N, Cravens, Petra C, Greenberg, Benjamin M, Vernino, Steven, Stüve, Olaf
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 03.12.2009; Elsevier
• Subjects: Advanced Basic Science; Animals; Autonomic Pathways - physiopathology; Biological and medical sciences; Cerebrospinal fluid. Meninges. Spinal cord; Disease Models, Animal; Encephalomyelitis, Autoimmune, Experimental - diagnosis; Encephalomyelitis, Autoimmune, Experimental - physiopathology; Experimental autoimmune encephalomyelitis; Female; Fundamental and applied biological sciences. Psychology; Iris - innervation; Iris - physiology; Male; Medical Education; Medical sciences; Mice; Mice, Inbred C57BL; Mouse models; Multiple sclerosis; Multiple Sclerosis - diagnosis; Multiple Sclerosis - physiopathology; Nervous system (semeiology, syndromes); Neurologic Examination - methods; Neurology; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ; Photic Stimulation; Pupil - physiology; Pupil Disorders - diagnosis; Pupil Disorders - physiopathology; Pupillometry; Reaction Time - physiology; Reference Values; Reflex, Pupillary - physiology; Sex Characteristics; Species Specificity; Vertebrates: nervous system and sense organs; Experimental autoimmune encephalomyelitis; Multiple sclerosis; Mouse models; Pupillometry; Animal model; Nervous system diseases; Encephalomyelitis; Rodentia; Cerebral disorder; Vertebrata; Mammalia; Mouse; Autonomic nervous system; Animal; Central nervous system disease; Pupillary reflex; Spinal cord disease
• ISSN: 1566-0702; 1872-7484
• DOI: 10.1016/j.autneu.2009.07.021

Abstract Pupillometry is a non-invasive technique, based on well-established neurophysiologic principles, that can be utilized to objectively characterize pathophysiologic demyelinating and neurodegenerative changes involving the pupillary reflex pathway. In animal models of human disorders, pupillometry derived reflex metrics could potentially be used to longitudinally monitor disease activity and responses to pharmacotherapies. These investigations would have important implications for translational initiatives focused on the identification and application of novel neuroprotective and restorative treatments for human diseases such as multiple sclerosis. Here, we have established normal reference values for various pupillary reflex metrics across different mouse strains. Ultimately, we anticipate that this new data will help to catalyze unique lines of inquiry using pupillometry methods.