A microsatellite map of wheat

• Published in: Genetics (Austin) Vol. 149; no. 4; pp. 2007 - 2023
• Main Authors: Roder, M.S. (IPK, Gaterleben, Germany.), Korzun, V, Wendehake, K, Plaschke, J, Tixier, M.H, Leroy, P, Ganal, M.W
• Format: Journal Article
• Language: English
• Published: United States Genetics Soc America 01.08.1998; Genetics Society of America
• Subjects: A GENOME; ADN; ANALYTICAL METHODS; B GENOME; Base Sequence; CARTE GENETIQUE; CHROMOSOME; CHROMOSOME MAPS; CHROMOSOMES; Chromosomes - genetics; CROMOSOMAS; D GENOME; DNA; DNA PRIMERS; DNA Primers - genetics; DNA, Plant - genetics; DNA, Plant - isolation & purification; Gene Library; GENE LOCATION; Genes; Genetic Linkage; GENETIC MAPPING; GENETIC MAPS; GENETIC MARKERS; Genetic Techniques; Genetics; GENOMAS; GENOME; GENOME ANALYSIS; Genome, Plant; GENOMES; LINKAGE GROUPS; LINKAGE MAPS; LOCALISATION DE GENE; LOCALIZACION DE GENES; LOCI; LOCUS; MAPAS GENETICOS; MARCADORES GENETICOS; MARQUEUR GENETIQUE; MICROSATELITES; MICROSATELLITE; Microsatellite Repeats; MICROSATELLITES; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Restriction Mapping; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; Triticum - genetics; TRITICUM AESTIVUM; Wheat
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/149.4.2007

Hexaploid bread wheat (Triticum aestivum L. em. Thell) is one of the world's most important crop plants and displays a very low level of intraspecific polymorphism. We report the development of highly polymorphic microsatellite markers using procedures optimized for the large wheat genome. The isolation of microsatellite-containing clones from hypomethylated regions of the wheat genome increased the proportion of useful markers almost twofold. The majority (80%) of primer sets developed are genome-specific and detect only a single locus in one of the three genomes of bread wheat (A, B, or D). Only 20% of the markers detect more than one locus. A total of 279 loci amplified by 230 primer sets were placed onto a genetic framework map composed of RFLPs previously mapped in the reference population of the International Triticeae Mapping Initiative (ITMI) Opata 85 x W7984. Sixty-five microsatellites were mapped at a LOD >2.5, and 214 microsatellites were assigned to the most likely intervals. Ninety-three loci were mapped to the A genome, 115 to the B genome, and 71 to the D genome. The markers are randomly distributed along the linkage map, with clustering in several centromeric regions.