Ultrastructural deformity of ovarian follicles induced by different cryopreservation protocols

Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demo...

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Published inFertility and sterility Vol. 94; no. 4; pp. 1548 - 1550.e1
Main Authors Kim, Gil Ah, M.S, Kim, Hwa Young, B.Sc, Kim, Jee Woong, M.S, Lee, Gene, D.D.S., Ph.D, Lee, Eunsong, D.V.M., Ph.D, Lim, Jeong Mook, D.V.M., Ph.D
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LanguageEnglish
Published New York, NY Elsevier Inc 01.09.2010
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Abstract Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demonstrated protocol specificity.
AbstractList Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demonstrated protocol specificity.
Author Kim, Jee Woong, M.S
Kim, Gil Ah, M.S
Lee, Gene, D.D.S., Ph.D
Lee, Eunsong, D.V.M., Ph.D
Kim, Hwa Young, B.Sc
Lim, Jeong Mook, D.V.M., Ph.D
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Issue 4
Keywords slow freezing
cryodamage
ultrastructure
Mouse ovary
vitrification
Gynecology
Vitrification
Rodentia
Obstetrics
Freezing
Ovary
Vertebrata
Mammalia
Mouse
Cryopreservation
Ultrastructure
Animal
Technique
Ovarian follicle
Language English
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Copyright (c) 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
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Snippet Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in...
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SubjectTerms Animals
Biological and medical sciences
cryodamage
Cryopreservation - methods
Female
Freezing - adverse effects
Gynecology. Andrology. Obstetrics
Internal Medicine
Medical sciences
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Mouse ovary
Obstetrics and Gynecology
Ovarian Follicle - pathology
Ovarian Follicle - ultrastructure
Ovary
slow freezing
ultrastructure
vitrification
Zona Pellucida - pathology
Zona Pellucida - ultrastructure
Title Ultrastructural deformity of ovarian follicles induced by different cryopreservation protocols
URI https://www.clinicalkey.es/playcontent/1-s2.0-S0015028209042150
https://dx.doi.org/10.1016/j.fertnstert.2009.12.029
https://www.ncbi.nlm.nih.gov/pubmed/20110089
https://search.proquest.com/docview/753997612
Volume 94
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