Ultrastructural deformity of ovarian follicles induced by different cryopreservation protocols

Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demo...

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Published inFertility and sterility Vol. 94; no. 4; pp. 1548 - 1550.e1
Main Authors Kim, Gil Ah, M.S, Kim, Hwa Young, B.Sc, Kim, Jee Woong, M.S, Lee, Gene, D.D.S., Ph.D, Lee, Eunsong, D.V.M., Ph.D, Lim, Jeong Mook, D.V.M., Ph.D
Format Journal Article
LanguageEnglish
Published New York, NY Elsevier Inc 01.09.2010
Elsevier
Subjects
Animals
Biological and medical sciences
cryodamage
Cryopreservation - methods
Female
Freezing - adverse effects
Gynecology. Andrology. Obstetrics
Internal Medicine
Medical sciences
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Mouse ovary
Obstetrics and Gynecology
Ovarian Follicle - pathology
Ovarian Follicle - ultrastructure
Ovary
slow freezing
ultrastructure
vitrification
Zona Pellucida - pathology
Zona Pellucida - ultrastructure
slow freezing
cryodamage
ultrastructure
Mouse ovary
vitrification
Gynecology
Vitrification
Rodentia
Obstetrics
Freezing
Ovary
Vertebrata
Mammalia
Mouse
Cryopreservation
Ultrastructure
Animal
Technique
Ovarian follicle
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Summary:Ultrastructural deformities were monitored after cryopreservation of F1 (B6CBAF1) mouse ovaries by either slow freezing or vitrification. Vacuole formation in the ooplasm, zona pellucida, and the cytoplasm of follicular cells, and mitochondrial deformity were detected. These types of cryodamage demonstrated protocol specificity.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Undefined-1
ObjectType-Feature-3
content type line 23
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2009.12.029