Unveiling the complexity of the maize transcriptome by single-molecule long-read sequencing

• Published in: Nature communications Vol. 7; no. 1; p. 11708
• Main Authors: Wang, Bo, Tseng, Elizabeth, Regulski, Michael, Clark, Tyson A, Hon, Ting, Jiao, Yinping, Lu, Zhenyuan, Olson, Andrew, Stein, Joshua C., Ware, Doreen
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.06.2016; Nature Publishing Group; Nature Portfolio
• Subjects: 38; 38/91; 631/337/2019; 631/449/1659; 631/61/514; Corn; DNA methylation; Gene Expression Profiling - methods; Gene Expression Regulation, Plant; Genes; Genomes; Humanities and Social Sciences; Insects; multidisciplinary; Plant Proteins - genetics; Plant Proteins - metabolism; Polymerase Chain Reaction; Science; Science (multidisciplinary); Sequence Analysis, RNA - methods; Transcriptome - genetics; Zea mays - genetics; New York; United States > US
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms11708

Zea mays is an important genetic model for elucidating transcriptional networks. Uncertainties about the complete structure of mRNA transcripts limit the progress of research in this system. Here, using single-molecule sequencing technology, we produce 111,151 transcripts from 6 tissues capturing ∼70% of the genes annotated in maize RefGen_v3 genome. A large proportion of transcripts (57%) represent novel, sometimes tissue-specific, isoforms of known genes and 3% correspond to novel gene loci. In other cases, the identified transcripts have improved existing gene models. Averaging across all six tissues, 90% of the splice junctions are supported by short reads from matched tissues. In addition, we identified a large number of novel long non-coding RNAs and fusion transcripts and found that DNA methylation plays an important role in generating various isoforms. Our results show that characterization of the maize B73 transcriptome is far from complete, and that maize gene expression is more complex than previously thought. Zea mays is an important crop species and genetic model but uncertainties remain regarding the structure of the transcriptome. Here Wang et al . use single-molecule sequencing and size-fractionated libraries to identify novel transcripts and isoforms illustrating the complexity of maize mRNA.