Morphology and efficiency of poly(styrene-co-divinylbenzene)-based monolithic capillary columns for the separation of small and large molecules

• Published in: Analytical and bioanalytical chemistry Vol. 400; no. 8; pp. 2391 - 2402
• Main Authors: Mohr, Jens H., Swart, Remco, Huber, Christian G.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.06.2011; Springer
• Subjects: Amino acids; Amino Acids - isolation & purification; Analytical Chemistry; Anti-Bacterial Agents - isolation & purification; beta-Lactams - isolation & purification; Biochemistry; Biopolymers; Capillarity; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography, High Pressure Liquid; Elution; Food Science; Globules; Laboratory Medicine; Microscopy, Electron, Scanning; Monitoring/Environmental Analysis; Morphology; Original Paper; Particle Size; Peptides; Permeability; Polystyrene resins; Polystyrenes - chemistry; Porosity; Separation; Surface Properties; Thyroid hormones; Thyroid Hormones - isolation & purification; Capillary columns; Proteins; Stationary phases; Peptides; β-Lactam antibiotics; Poly(styrene-divinylbenzene); Monolithic morphology; Thyroid hormones; Monolithic columns; Dansylated amino acids; HPLC
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-011-4777-x

The morphology of organic monolithic stationary phases based on poly(styrene-divinylbenzene) was modified by changing the ratio of monomers to microporogen in order to make them also suitable for small molecule separations. The morphology of the columns was characterized by high-resolution scanning electron micrography, showing larger primary globules and larger macropores, as well as no mesopores >20 nm in the monolithic skeleton. The permeability of the modified monoliths was approximately three times higher than that of columns which have been optimized for large molecule separations, enabling operation of a 30 cm long column at pressures below 250 bar. In the isocratic separation of dansylated amino acids, plate counts of 50000–107000 m −1 were achievable, which are equivalent to efficiencies obtained with 3.1 μm porous particles. The separation performance for small molecules in gradient elution was investigated using mixtures of dansylated amino acids, β-lactam antibiotics, and thyroid hormones. Finally, the modified monolithic capillary columns also proved to be highly efficient in the separation of biopolymers such as peptides and proteins, enabling peak width at half height of 3–8 s and peak capacities of 110–180 in 15–30 min gradient runs.