Identification of Rice AI-responsive Genes by Semi-quantitative Polymerase Chain Reaction using Sulfite Reductase as a Novel Endogenous Control

• Published in: Journal of integrative plant biology Vol. 52; no. 5; pp. 505 - 514
• Main Author: Jianjun Zhang Ying Yin Yuqi Wang Xinxiang Peng
• Format: Journal Article
• Language: English
• Published: Laboratory of Molecular Plant Physiology,College of Life Sciences,South China Agricultural University,Guangzhou 510642,China 2010; Key Laboratory of Plant Functional Genomics and Biotechnology,Education Department of Guangdong Province,College of Life Sciences,South China Agricultural University,Guangzhou 510642,China%Laboratory of Molecular Plant Physiology,College of Life Sciences,South China Agricultural University,Guangzhou 510642,China%Sciences,South China Agricultural University,Guangzhou 510642,China
• Subjects: PCR分析; 亚硫酸盐还原酶; 半定量; 水稻基因; 聚合酶链反应
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/j.1744-7909.2010.00931.x

Based on the evidence that Al resistance is an inducible process and rice is an Al-resistant crop, identification of Al-responsive genes from rice may help to further clone Al.resistant genes in plants. Semi-quantitative and real-time polymerase chain reaction （PCR） is widely applied in gene transcrip- tional analyses, particularly for those genes with low transcript abundance. Normalization with proper endogenous control （EC） genes is critical for these two approaches in terms of reliability and precision. We first noticed that the expression of several commonly-used EC genes was depressed under AI stress, while sulfite reductase gene （SR） was stable throughout the AI treatment. The reliability of SR as an EC gene was further tested by analyzing the expression of a number of genes in response to Al challenge. Except for the consistent results obtained for the four previously-identified genes, nine additional genes were newly defined as Al-responsive in this study. Collectively, our results suggest that SR can be used as a novel EC gene for semi-quantitative and real-time PCR analysis of Al responsive genes, and that activated transport of silicon and stimulated metabolism of carotenoid and terpenoid could be involved in Al resistance in rice plants.