酒精依赖对地西泮抗惊厥作用的影响

目的：研究酒精依赖对地西泮抗惊厥作用的影响。方法成年昆明种小鼠36只，均分为酒精依赖组（A组）、地西泮组（D组）和生理盐水组（N组）。A组用含0.8％乙醇的生理盐水进行腹腔注射，其他2组注射生理盐水，每天上、下午各1次，0.2 mL/次，注射7 d。检测小鼠7 d的自主活动情况。7 d后行电惊厥实验，A组和D组腹腔注射0.05%地西泮溶液0.05 mL/10 g，N组注射等体积生理盐水，给药前及给药后15、30、60 min分别测量3组小鼠惊厥阈值。结果第2～6天，A组小鼠自主活动计数小于其他2组（P＜0.05），第1和7天，3组小鼠自主活动计数差异无统计学意义。给药前，A组惊厥阈值高于D组和...

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Published in天津医药 Vol. 43; no. 4; pp. 360 - 363
Main Author 汤洁 王朵朵 马焦 凤雅薇 楼鹏 张心宇 徐佳晨 高洋洋 武静茹
Format Journal Article
LanguageChinese
Published 徐州医学院麻醉学院2011级2班 邮编221000%徐州医学院麻醉生理学教研室 2015
Subjects
γ-氨基丁酸
地西泮
抗惊厥
电刺激
酒精依赖
electric stimulation
anticonvulsant action
alcohol dependence
diazepam
γ-氨基丁酸
地西泮
酒精依赖
电刺激
gamma-aminobutyric acid
抗惊厥
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Summary:目的：研究酒精依赖对地西泮抗惊厥作用的影响。方法成年昆明种小鼠36只，均分为酒精依赖组（A组）、地西泮组（D组）和生理盐水组（N组）。A组用含0.8％乙醇的生理盐水进行腹腔注射，其他2组注射生理盐水，每天上、下午各1次，0.2 mL/次，注射7 d。检测小鼠7 d的自主活动情况。7 d后行电惊厥实验，A组和D组腹腔注射0.05%地西泮溶液0.05 mL/10 g，N组注射等体积生理盐水，给药前及给药后15、30、60 min分别测量3组小鼠惊厥阈值。结果第2～6天，A组小鼠自主活动计数小于其他2组（P＜0.05），第1和7天，3组小鼠自主活动计数差异无统计学意义。给药前，A组惊厥阈值高于D组和N组（P＜0.05）；给药后，N组惊厥阈值与给药前相比差异无统计学意义；给药后15 min，D组惊厥阈值高于A组和N组，A组阈值高于N组（P＜0.05）；给药后30 min、60 min，A组和D组惊厥阈值均高于N组（P＜0.05），A组和D组惊厥阈值差异无统计学意义。结论酒精依赖有抗惊厥的作用；酒精依赖会使地西泮的抗惊厥作用减弱。
Bibliography:Objective To study the impacts of alcohol dependence on the anticonvulsant effect of diazepam. Meth？ods Kunming mice （n=36） were divided into 3 groups （n=12 in each group）, Alcohol Dependence Group（A group）, Diaze？pam Group（D group）and Normal Saline Group（N group）. A group received an intraperitoneal injection with a 0.2 mL dose of 0.8%alcohol in NS （normal saline） , while both D and N group received an injection with a 0.2 mL dose of NS without alco？hol , twice a day. Mice’s autonomic activities were monitored every day. After 7 days, the electroconvulsive experiment was performed. Both A and D group were given a weight-based dose of 0.05 mL/10 g of 0.05%diazepam via intraperitoneal injec？ tion, while N group was given a 0.05 mL/10 g dose of NS. Before administration and after 15, 30, 60 min of administration, the convulsion threshold of each group was measured. Results The count of autonomic activity of mice in A group was less than that of mice in D and N group during the 2nd day to 6th day（P〈0.05）. On the
ISSN:0253-9896
DOI:10.11958/j.issn.0253-9896.2015.04.007