结肠癌患者血清中microRNA-183及胸苷激酶1的表达及意义
目的检测结肠癌患者血清中mi RNA-183及胸苷激酶1（TK1）的表达并探讨其临床意义。方法收集结肠癌患者及健康体检者血清标本各52例,荧光定量PCR检测mi RNA-183表达,免疫印迹增强化学发光法检测TK1的表达,分析两者之间的相关性及与临床病理的关系。结果与正常对照组相比,结肠癌患者血清mi R-183的表达相对升高（P〈0.01）;中低分化腺癌者血清mi R-183的表达量高于高分化腺癌（P〈0.01）;Ⅲ~Ⅳ期结肠癌者血清中mi R-183的表达量高于Ⅰ~Ⅱ期（P〈0.01）,且在有淋巴结转移的患者中表达较高（P〈0.01）;受试者工作特征（ROC）曲线显示,以相对表达量1.15...
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| Published in | 天津医药 Vol. 45; no. 1; pp. 72 - 75 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
天津医科大学三中心临床学院 邮编300170%天津市第三中心医院
2017
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-9896 |
| DOI | 10.11958/20161119 |
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| Abstract | 目的检测结肠癌患者血清中mi RNA-183及胸苷激酶1（TK1）的表达并探讨其临床意义。方法收集结肠癌患者及健康体检者血清标本各52例,荧光定量PCR检测mi RNA-183表达,免疫印迹增强化学发光法检测TK1的表达,分析两者之间的相关性及与临床病理的关系。结果与正常对照组相比,结肠癌患者血清mi R-183的表达相对升高（P〈0.01）;中低分化腺癌者血清mi R-183的表达量高于高分化腺癌（P〈0.01）;Ⅲ~Ⅳ期结肠癌者血清中mi R-183的表达量高于Ⅰ~Ⅱ期（P〈0.01）,且在有淋巴结转移的患者中表达较高（P〈0.01）;受试者工作特征（ROC）曲线显示,以相对表达量1.15为临界点,血清mi R-183可能具有诊断结肠癌的价值,其敏感度为78.8%,特异度为67.3%,阳性预测值为78.9%,阴性预测值为70.2%。结肠癌患者血清中TK1的表达也较正常对照组升高,中低分化腺癌高于高分化腺癌（P〈0.05）,Ⅲ~Ⅳ期高于Ⅰ~Ⅱ期（P〈0.01）。血清中mi R-183与TK1的表达呈正相关（rs=0.692,P〈0.01）。结论血清中mi R-183及TK1可能是早期结肠癌的诊断标志物,并可用于判断肿瘤的恶性程度及预后。 |
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| AbstractList | R735.35; 目的：检测结肠癌患者血清中miRNA-183及胸苷激酶1（TK1）的表达并探讨其临床意义。方法收集结肠癌患者及健康体检者血清标本各52例，荧光定量PCR检测miRNA-183表达，免疫印迹增强化学发光法检测TK1的表达，分析两者之间的相关性及与临床病理的关系。结果与正常对照组相比，结肠癌患者血清miR-183的表达相对升高（P<0.01）；中低分化腺癌者血清miR-183的表达量高于高分化腺癌（P<0.01）；Ⅲ～Ⅳ期结肠癌者血清中miR-183的表达量高于Ⅰ～Ⅱ期（P<0.01），且在有淋巴结转移的患者中表达较高（P<0.01）；受试者工作特征（ROC）曲线显示，以相对表达量1.15为临界点，血清miR-183可能具有诊断结肠癌的价值，其敏感度为78.8%，特异度为67.3%，阳性预测值为78.9%，阴性预测值为70.2%。结肠癌患者血清中TK1的表达也较正常对照组升高，中低分化腺癌高于高分化腺癌（P<0.05），Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期（P<0.01）。血清中miR-183与TK1的表达呈正相关（rs=0.692，P＜0.01）。结论血清中miR-183及TK1可能是早期结肠癌的诊断标志物，并可用于判断肿瘤的恶性程度及预后。 目的检测结肠癌患者血清中mi RNA-183及胸苷激酶1（TK1）的表达并探讨其临床意义。方法收集结肠癌患者及健康体检者血清标本各52例,荧光定量PCR检测mi RNA-183表达,免疫印迹增强化学发光法检测TK1的表达,分析两者之间的相关性及与临床病理的关系。结果与正常对照组相比,结肠癌患者血清mi R-183的表达相对升高（P〈0.01）;中低分化腺癌者血清mi R-183的表达量高于高分化腺癌（P〈0.01）;Ⅲ~Ⅳ期结肠癌者血清中mi R-183的表达量高于Ⅰ~Ⅱ期（P〈0.01）,且在有淋巴结转移的患者中表达较高（P〈0.01）;受试者工作特征（ROC）曲线显示,以相对表达量1.15为临界点,血清mi R-183可能具有诊断结肠癌的价值,其敏感度为78.8%,特异度为67.3%,阳性预测值为78.9%,阴性预测值为70.2%。结肠癌患者血清中TK1的表达也较正常对照组升高,中低分化腺癌高于高分化腺癌（P〈0.05）,Ⅲ~Ⅳ期高于Ⅰ~Ⅱ期（P〈0.01）。血清中mi R-183与TK1的表达呈正相关（rs=0.692,P〈0.01）。结论血清中mi R-183及TK1可能是早期结肠癌的诊断标志物,并可用于判断肿瘤的恶性程度及预后。 |
| Abstract_FL | Objective To detect the expression and significance of serum miRNA-183 and TK1 in patients with colorectal cancer, and the mechanism thereof. Methods Fifty-two serum samples of colorectal cancer patients and paired health serum samples were collected. The expression of miRNA-183 was detected by real-time quantitative PCR, and TK1 was detected by Western blot enhanced chemiluminescence assay. The correlation between miRNA-183 and TK1 and their relations with the clinicpathologic characteristics were analyzed. Results The serum miRNA-183 expression was significantly higher in colorectal cancer group than that in normal control group (P<0.01). The expression of serum miRNA-183 was significantly higher in stage Ⅲ-Ⅳ group than that in stage Ⅰ-Ⅱ group (P < 0.01). There was more significant increase in serum miRNA-183 in lymphatic metastasis group than that without lymphatic metastasis group (P < 0.01). Receiver operating characteristic (ROC) curve showed that of there was a diagnostic value for serum miRNA-183 in colorectal cancer, with an optimal value of 1.15. The diagnostic sensitivity and specificity were 78.8%and 67.3%, and the positive predictive value and negative predictive value were 78.9%and 70.2%. The serum TK1 expression was also higher in colorectal cancer group compared with that of normal control group (P<0.01). And the TK1 expression was also higher in stageⅢ-Ⅳgroup than that in stageⅠ-Ⅱgroup (P<0.01). Furthermore, miRNA-183 expression was positively correlated with TK1 expression (rs=0.692, P<0.01). Conclusion The serum expression levels of miR-183 and TK1 may act as tumor markers for early colorectal cancer diagnosis, and also can be used to predict the malignant degree and prognosis of colorectal cancer. |
| Author | 王金亮 孟祥朝 张自立 李琦 |
| AuthorAffiliation | 天津医科大学三中心临床学院,300170 天津市第三中心医院 |
| AuthorAffiliation_xml | – name: 天津医科大学三中心临床学院 邮编300170%天津市第三中心医院 |
| Author_FL | ZHANG Zi-li LI Qi WANG Jin-liang MENG Xiang-chao |
| Author_FL_xml | – sequence: 1 fullname: WANG Jin-liang – sequence: 2 fullname: MENG Xiang-chao – sequence: 3 fullname: ZHANG Zi-li – sequence: 4 fullname: LI Qi |
| Author_xml | – sequence: 1 fullname: 王金亮 孟祥朝 张自立 李琦 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | The expression and significance of serum microRNA-183 and TK1 in patients with colorectal cancer |
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| Keywords | microRNAs colonic neoplasms 结肠癌 血清 thymidine kinase colon cancer miRNA-183 miR-183 结肠肿瘤 胸苷激酶 serum 微RNAs |
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| Notes | Objective To detect the expression and significance of serum miRNA- 183 and TK1 in patients withcolorectal cancer, and the mechanism thereof. Methods Fifty-two serum samples of colorectal cancer patients and pairedhealth serum samples were collected. The expression of miRNA-183 was detected by real-time quantitative PCR, and TK1 was detected by Western blot enhanced chemiluminescence assay. The correlation between miRNA-183 and TK1 and theirrelations with the clinicpathologic characteristics were analyzed. Results The serum miRNA- 183 expression wassignificantly higher in colorectal cancer group than that in normal control group（P 〈 0.01）. The expression of serum miRNA-183 was significantly higher in stage Ⅲ-Ⅳ group than that in stage Ⅰ-Ⅱ group（P 〈 0.01）. There was more significantincrease in serum miRNA- 183 in lymphatic metastasis group than that without lymphatic metastasis group（P 〈 0.01）.Receiver operating characteristic（ROC） curve showed that of there was a diagnostic value for serum miRNA- 183 incolore |
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| Snippet | 目的检测结肠癌患者血清中mi RNA-183及胸苷激酶1（TK1）的表达并探讨其临床意义。方法收集结肠癌患者及健康体检者血清标本各52例,荧光定量PCR检测mi RNA-183表达,免疫印迹增强化学发光法检测TK1的表达,分析两者之间的相关性及与临床病理的关系。结果与正常对照组相比,结肠癌患者血清mi... R735.35;... |
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| SubjectTerms | miRNA-183 微RNAs 结肠癌 结肠肿瘤 胸苷激酶 血清 |
| Title | 结肠癌患者血清中microRNA-183及胸苷激酶1的表达及意义 |
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