结肠癌患者血清中microRNA-183及胸苷激酶1的表达及意义

目的检测结肠癌患者血清中mi RNA-183及胸苷激酶1（TK1）的表达并探讨其临床意义。方法收集结肠癌患者及健康体检者血清标本各52例,荧光定量PCR检测mi RNA-183表达,免疫印迹增强化学发光法检测TK1的表达,分析两者之间的相关性及与临床病理的关系。结果与正常对照组相比,结肠癌患者血清mi R-183的表达相对升高（P〈0.01）;中低分化腺癌者血清mi R-183的表达量高于高分化腺癌（P〈0.01）;Ⅲ~Ⅳ期结肠癌者血清中mi R-183的表达量高于Ⅰ~Ⅱ期（P〈0.01）,且在有淋巴结转移的患者中表达较高（P〈0.01）;受试者工作特征（ROC）曲线显示,以相对表达量1.15...

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Published in天津医药 Vol. 45; no. 1; pp. 72 - 75
Main Author 王金亮 孟祥朝 张自立 李琦
Format Journal Article
LanguageChinese
Published 天津医科大学三中心临床学院 邮编300170%天津市第三中心医院 2017
Subjects
miRNA-183
微RNAs
结肠癌
结肠肿瘤
胸苷激酶
血清
microRNAs
colonic neoplasms
结肠癌
血清
thymidine kinase
colon cancer
miRNA-183
miR-183
结肠肿瘤
胸苷激酶
serum
微RNAs
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ISSN0253-9896
DOI10.11958/20161119

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Summary:目的检测结肠癌患者血清中mi RNA-183及胸苷激酶1（TK1）的表达并探讨其临床意义。方法收集结肠癌患者及健康体检者血清标本各52例,荧光定量PCR检测mi RNA-183表达,免疫印迹增强化学发光法检测TK1的表达,分析两者之间的相关性及与临床病理的关系。结果与正常对照组相比,结肠癌患者血清mi R-183的表达相对升高（P〈0.01）;中低分化腺癌者血清mi R-183的表达量高于高分化腺癌（P〈0.01）;Ⅲ~Ⅳ期结肠癌者血清中mi R-183的表达量高于Ⅰ~Ⅱ期（P〈0.01）,且在有淋巴结转移的患者中表达较高（P〈0.01）;受试者工作特征（ROC）曲线显示,以相对表达量1.15为临界点,血清mi R-183可能具有诊断结肠癌的价值,其敏感度为78.8%,特异度为67.3%,阳性预测值为78.9%,阴性预测值为70.2%。结肠癌患者血清中TK1的表达也较正常对照组升高,中低分化腺癌高于高分化腺癌（P〈0.05）,Ⅲ~Ⅳ期高于Ⅰ~Ⅱ期（P〈0.01）。血清中mi R-183与TK1的表达呈正相关（rs=0.692,P〈0.01）。结论血清中mi R-183及TK1可能是早期结肠癌的诊断标志物,并可用于判断肿瘤的恶性程度及预后。
Bibliography:Objective To detect the expression and significance of serum miRNA- 183 and TK1 in patients withcolorectal cancer, and the mechanism thereof. Methods Fifty-two serum samples of colorectal cancer patients and pairedhealth serum samples were collected. The expression of miRNA-183 was detected by real-time quantitative PCR, and TK1 was detected by Western blot enhanced chemiluminescence assay. The correlation between miRNA-183 and TK1 and theirrelations with the clinicpathologic characteristics were analyzed. Results The serum miRNA- 183 expression wassignificantly higher in colorectal cancer group than that in normal control group（P 〈 0.01）. The expression of serum miRNA-183 was significantly higher in stage Ⅲ-Ⅳ group than that in stage Ⅰ-Ⅱ group（P 〈 0.01）. There was more significantincrease in serum miRNA- 183 in lymphatic metastasis group than that without lymphatic metastasis group（P 〈 0.01）.Receiver operating characteristic（ROC） curve showed that of there was a diagnostic value for serum miRNA- 183 incolore
ISSN:0253-9896
DOI:10.11958/20161119