An overview on application of phage display technique in immunological studies

Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of mono-clonal antibody, peptides, peptidomimetics and epitope mapping are main application of phage display. Selection of monoclonal anti...

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Published inAsian Pacific journal of tropical biomedicine Vol. 7; no. 7; pp. 599 - 602
Main Author Abbas Rami Mahdi Behdani Najmeh Yardehnavi Mahdi Habibi-Anbouhi Fatemeh Kazemi-Lomedasht
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.07.2017
Biotechnology Research Center, Venom&Biotherapeutics Molecules Lab., Pasteur Institute of Iran, Tehran, Iran%Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Department of Medical Biotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran%National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
Wolters Kluwer Medknow Publications
Subjects
Antibody
Antibody library
Epitope mapping
Immunology
Peptide library
Phage display
Antibody library
Epitope mapping
Phage display
Peptide library
Immunology
Antibody
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Summary:Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of mono-clonal antibody, peptides, peptidomimetics and epitope mapping are main application of phage display. Selection of monoclonal antibody or peptides that are displayed on the surface of the phages can be occurred through biopanning process. In biopanning process phage library is incubated with antigen and particular phages can be identified and iso-lated. Increasing the stringency in the biopanning rounds can be help to select phages with high affinity and specificity. Here, we describe an overview of phage display application with focusing on monoclonal antibody production and epitope mapping.
Bibliography:Abbas Rami[1];Mahdi Behdani[1];Najmeh Yardehnavi[2];Mahdi Habibi-Anbouhi[3];Fatemeh Kazemi-Lomedasht[1]
Phage display;Antibody;Epitope mapping;Immunology;Antibody library;Peptide library
Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of mono-clonal antibody, peptides, peptidomimetics and epitope mapping are main application of phage display. Selection of monoclonal antibody or peptides that are displayed on the surface of the phages can be occurred through biopanning process. In biopanning process phage library is incubated with antigen and particular phages can be identified and iso-lated. Increasing the stringency in the biopanning rounds can be help to select phages with high affinity and specificity. Here, we describe an overview of phage display application with focusing on monoclonal antibody production and epitope mapping.
ISSN:2221-1691
2588-9222
DOI:10.1016/j.apjtb.2017.06.001