Light-inducible carotenoid production controlled by a MarR-type regulator in Corynebacterium glutamicum

Carotenoid production in some non-phototropic bacteria occurs in a light-dependent manner to protect cells from photo-oxidants. Knowledge regarding the transcriptional regulator involved in the light-dependent production of carotenoids of non-phototrophic bacteria has been mainly confined to coenzym...

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Published inScientific reports Vol. 9; no. 1; pp. 13136 - 15
Main Authors Sumi, Satoru, Suzuki, Yuto, Matsuki, Tetsuro, Yamamoto, Takahiro, Tsuruta, Yudai, Mise, Kou, Kawamura, Takuya, Ito, Yusuke, Shimada, Yuka, Watanabe, Erika, Watanabe, Shoko, Toriyabe, Minami, Takano (Shiratori), Hatsumi, Ueda, Kenji, Takano, Hideaki
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 11.09.2019
Nature Publishing Group
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Summary:Carotenoid production in some non-phototropic bacteria occurs in a light-dependent manner to protect cells from photo-oxidants. Knowledge regarding the transcriptional regulator involved in the light-dependent production of carotenoids of non-phototrophic bacteria has been mainly confined to coenzyme B 12 -based photo-sensitive regulator CarH/LitR family proteins belonging to a MerR family transcriptional regulator. In this study, we found that bacteria belonging to Micrococcales and Corynebacteriales exhibit light-dependent carotenoid-like pigment production including an amino acid-producer Corynebacterium glutamicum AJ1511. CrtR is a putative MarR family transcriptional regulator located in the divergent region of a carotenoid biosynthesis gene cluster in the genome of those bacteria. A null mutant for crtR of C . glutamicum AJ1511 exhibited constitutive production of carotenoids independent of light. A complemented strain of the crtR mutant produced carotenoids in a light-dependent manner. Transcriptional analysis revealed that the expression of carotenoid biosynthesis genes is regulated in a light-dependent manner in the wild type, while the transcription was upregulated in the crtR mutant irrespective of light. In vitro experiments demonstrated that a recombinant CrtR protein binds to the specific sequences within the intergenic region of crtR and crtE , which corresponds to −58 to −7 for crtE , and +26 to −28 for crtR with respect to the transcriptional start site, and serves as a repressor for crtE transcription directed by RNA polymerase containing SigA. Taken together, the results indicate that CrtR light-dependently controls the expression of the carotenoid gene cluster in C . glutamicum and probably closely related Actinobacteria .
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-49384-7