Down regulation of p-coumarate 3-hydroxylase in petunia uniquely alters the profile of emitted floral volatiles

• Published in: Scientific reports Vol. 9; no. 1; pp. 8852 - 12
• Main Authors: Kim, Joo Young, Swanson, Robert T., Alvarez, Maria I., Johnson, Timothy S., Cho, Keun H., Clark, David G., Colquhoun, Thomas A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 20.06.2019; Nature Publishing Group
• Subjects: 38; 38/22; 38/23; 38/89; 38/90; 631/449/1736; 631/45/320; 96/106; 96/44; Accumulation; Acid production; Acyltransferases; Biosynthesis; Carboxylic Ester Hydrolases; Cinnamic acid; Coumaric acid; Down-Regulation; Emissions; Esterase; Eugenol; Flowers; Flowers - metabolism; Food irradiation; Humanities and Social Sciences; Hydroxylase; Isoeugenol; Mixed Function Oxygenases - metabolism; multidisciplinary; p-Coumaric acid; Petunia - enzymology; Phenylalanine; Propionates - chemistry; Science; Science (multidisciplinary); Transcription; Volatile Organic Compounds - metabolism; Volatiles; Volatilization
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-45183-2

Petunia × hybrida cv ‘Mitchell Diploid’ floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis ultimately produces floral volatiles derived sequentially from phenylalanine, cinnamic acid, and p -coumaric acid. In an attempt to better understand biochemical steps after p -coumaric acid production, we cloned and characterized three petunia transcripts with high similarity to p-coumarate 3-hydroxylase ( C3H ), hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyl transferase ( HCT ), and caffeoyl shikimate esterase ( CSE ). Transcript accumulation of PhC3H and PhHCT was highest in flower limb tissue during open flower stages. PhCSE transcript accumulation was also highest in flower limb tissue, but it was detected earlier at initial flower opening with a bell-shaped distribution pattern. Down regulation of endogenous PhC3H transcript resulted in altered transcript accumulation of many other FVBP network transcripts, a reduction in floral volatiles, and the emission of a novel floral volatile. Down regulation of PhHCT transcript did not have as large of an effect on floral volatiles as was observed for PhC3H down regulation, but eugenol and isoeugenol emissions were significantly reduced on the downstream floral volatiles. Together these results indicate that PhC3H is involved in FVBP biosynthesis and the reduction of PhC3H transcript influences FVBP metabolism at the network level. Additional research is required to illustrate PhHCT and PhCSE functions of petunia.