The UDP-glucose pyrophosphorylase from Giardia lamblia is redox regulated and exhibits promiscuity to use galactose-1-phosphate

• Published in: Biochimica et biophysica acta Vol. 1850; no. 1; pp. 88 - 96
• Main Authors: Ebrecht, Ana C., Asención Diez, Matías D., Piattoni, Claudia V., Guerrero, Sergio A., Iglesias, Alberto A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2015
• Subjects: Amino Acid Sequence; Biocatalysis; Carbohydrate metabolism; catalytic activity; Cloning, Molecular; cysteine; Cysteine - metabolism; DNA; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli - genetics; Galactose-1-phosphate; Galactosephosphates - metabolism; Giardia lamblia; Giardia lamblia - enzymology; Giardia lamblia - genetics; Glucose-1-phosphate; Glucosephosphates - metabolism; glycogen; heterologous gene expression; humans; hydrogen peroxide; Kinetics; metabolites; molecular cloning; Molecular Sequence Data; nitric oxide; oxidation; Oxidation-Reduction; parasites; pathogenicity; pathogens; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Recombinant Proteins - metabolism; Redox regulated; Sequence Homology, Amino Acid; Substrate Specificity; Thioredoxins - metabolism; Time Factors; transferases; UDP-glucose pyrophosphorylase; uridine triphosphate; UTP-Glucose-1-Phosphate Uridylyltransferase - genetics; UTP-Glucose-1-Phosphate Uridylyltransferase - metabolism; UDP-glucose pyrophosphorylase; Carbohydrate metabolism; Galactose-1-phosphate; Glucose-1-phosphate; Redox regulated

Giardia lamblia is a pathogen of humans and other vertebrates. The synthesis of glycogen and of structural oligo and polysaccharides critically determine the parasite's capacity for survival and pathogenicity. These characteristics establish that UDP-glucose is a relevant metabolite, as it is a...